ArticleEmerging infectious diseases2015
Nanomicroarray and multiplex next-generation sequencing for simultaneous identification and characterization of influenza viruses.
Article in Emerging infectious diseases, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Review
- Review
- Review
- Viral genome sequencing methods: benefits and pitfalls of current approaches.Biochemical Society transactions · 2024Review
- Epidemiology and diagnosis technologies of human metapneumovirus in China: a mini review.Virology journal · 2024Review
- Universal primers for rift valley fever virus whole-genome sequencing.Scientific reports · 2023Article
- LAMP-Based Point-of-Care Biosensors for Rapid Pathogen Detection.Biosensors · 2022Review
- Enhancing global health security in Thailand: Strengths and challenges of initiating a One Health approach to avian influenza surveillance.One health (Amsterdam, Netherlands) · 2022Article
- A computational toolset for rapid identification of SARS-CoV-2, other viruses and microorganisms from sequencing data.Briefings in bioinformatics · 2021Article
- Graphene functionalized field-effect transistors for ultrasensitive detection of Japanese encephalitis and Avian influenza virus.Scientific reports · 2020Article
- Advances in laboratory assays for detecting human metapneumovirus.Annals of translational medicine · 2020Review
- Application of magnetic nanoparticles in nucleic acid detection.Journal of nanobiotechnology · 2020Review
- Recent advances in the detection of respiratory virus infection in humans.Journal of medical virology · 2020Review
- The difference of detection rate of avian influenza virus in the wild bird surveillance using various methods.Journal of veterinary science · 2019Article
- Direct RNA Sequencing of the Coding Complete Influenza A Virus Genome.Scientific reports · 2018Article
- An Improved Barcoded Oligonucleotide Primers-based Next-generation Sequencing Approach for Direct Identification of Viral Pathogens in Clinical Specimens.Biomedical and environmental sciences : BES · 2017Article
- Review
- Article
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Conventional methods for detection and discrimination of influenza viruses are time consuming and labor intensive. We developed a diagnostic platform for simultaneous identification and characterization of influenza viruses that uses a combination of nanomicroarray for screening and multiplex next-generation sequencing (NGS) assays for laboratory confirmation. The nanomicroarray was developed to target hemagglutinin, neuraminidase, and matrix genes to identify influenza A and B viruses. PCR amplicons synthesized by using an adapted universal primer for all 8 gene segments of 9 influenza A subtypes were detected in the nanomicroarray and confirmed by the NGS assays. This platform can simultaneously detect and differentiate multiple influenza A subtypes in a single sample. Use of these methods as part of a new diagnostic algorithm for detection and confirmation of influenza infections may provide ongoing public health benefits by assisting with future epidemiologic studies and improving preparedness for potential influenza pandemics.
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