ArticleNature protocols2015
Supercooling preservation and transplantation of the rat liver.
Article in Nature protocols, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
50 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Optimizing Livers for Transplantation Using Machine Perfusion versus Cold Storage in Large Animal Studies and Human Studies: A Systematic Review and Meta-Analysis.BioMed research international · 2018Pooled it
- Dual acellular subnormothermic machine perfusion for enhanced uterus preservation in a swine model.Acta obstetricia et gynecologica Scandinavica · 2026Article
- Protective effect of Caftaric Acid against hepatic cold ischemia/reperfusion injury in the liver.Biology direct · 2026Article
- Reduction in Red Blood Cell Lysis by Polymer Intervention During Rodent Liver Normothermic Machine Perfusion.Transplantation direct · 2026Article
- Mitochondrial hyperoxidation contributes to warm ischemia-reperfusion injury in rat and pig livers.Communications medicine · 2026Article
- Article
- Small animalWorld journal of gastroenterology · 2026Review
- Cryopreservation of biological materials: applications and economic perspectives.In vitro cellular & developmental biology. Animal · 2026Review
- Cellular and Molecular Changes Induced by Various Preservation Temperatures and Methods of Preservation in Renal Grafts and Other Solid Organ Grafts.International journal of molecular sciences · 2026Review
- Anti-Icing Organogel Enables Quasi-Homogeneous Supercooling Preservation of Mouse Hearts.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- A simplified and reproducibleLiver research (Beijing, China) · 2025Article
- Tissue Preservation and Access: Modern Innovation in Biobanking Moving Forwards a Personalized Treatment.Journal of personalized medicine · 2025Review
- A simplified computational liver perfusion model, with applications to organ preservation.Scientific reports · 2025Article
- Organ preservation: current limitations and optimization approaches.Frontiers in medicine · 2025Review
- Beyond the icebox: modern strategies in organ preservation for transplantation.Clinical transplantation and research · 2024Review
- [Advances and perspectives in vascularized composite allotransplantation preservation].Bulletin de l'Academie nationale de medecine · 2024Article
- Optimized partial freezing protocol enables 10-day storage of rat livers.Scientific reports · 2024Article
- Supercooling preservation of vascularized composite allografts through CPA optimization, thermal tracking, and stepwise loading techniques.Scientific reports · 2024Article
- Sub-zero non-freezing of vascularized composite allografts in a rodent partial hindlimb model.Cryobiology · 2024Article
- Optimized Partial Freezing Protocol Enables 10-Day Storage of Rat Livers.Research square · 2024Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
Abstract
The current standard for liver preservation involves cooling of the organ on ice (0-4 °C). Although it is successful for shorter durations, this method of preservation does not allow long-term storage of the liver. The gradual loss of hepatic viability during preservation puts pressure on organ sharing and allocation, may limit the use of suboptimal grafts and necessitates rushed transplantation to achieve desirable post-transplantation outcomes. In an attempt to improve and prolong liver viability during storage, alternative preservation methods are under investigation. For instance, ex vivo machine perfusion systems aim to sustain and even improve viability by supporting hepatic function at warm temperatures, rather than simply slowing down deterioration by cooling. Here we describe a novel subzero preservation technique that combines ex vivo machine perfusion with cryoprotectants to facilitate long-term supercooled preservation. The technique improves the preservation of rat livers to prolong storage times as much as threefold, which is validated by successful long-term recipient survival after orthotopic transplantation. This protocol describes how to load rat livers with cryoprotectants to prevent both intracellular and extracellular ice formation and to protect against hypothermic injury. Cryoprotectants are loaded ex vivo using subnormothermic machine perfusion (SNMP), after which livers can be cooled to -6 °C without freezing and kept viable for up to 96 h. Cooling to a supercooled state is controlled, followed by 3 h of SNMP recovery and orthotopic liver transplantation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.