ArticlePloS one2015
A cell-permeable fluorescent polymeric thermometer for intracellular temperature mapping in mammalian cell lines.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
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Who cites it
53 citing papers in PubMed.
- Millikelvin Intracellular Nanothermometry with Nanodiamonds.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Genetically-encoded temperature indicators for thermal biology.Biophysics and physicobiology · 2025Article
- Mitochondria: great balls of fire.The FEBS journal · 2024Review
- Heat application in live cell imaging.FEBS open bio · 2024Review
- Naltrexone blocks alcohol-induced effects on kappa-opioid receptors in the plasma membrane.Translational psychiatry · 2024Article
- TRPV2: a universal regulator in cellular physiology with a yet poorly defined thermosensitivity.The journal of physiological sciences : JPS · 2024Review
- Mitochondrial Thermogenesis Can Trigger Heat Shock Response in the Nucleus.ACS central science · 2024Article
- Implication of thermal signaling in neuronal differentiation revealed by manipulation and measurement of intracellular temperature.Nature communications · 2024Article
- Temperature-sensing riboceptors.RNA biology · 2024Article
- Natural convection in the cytoplasm: Theoretical predictions of buoyancy-driven flows inside a cell.PloS one · 2024Article
- Cellular Thermal Biology Using Fluorescent Nanothermometers.Advances in experimental medicine and biology · 2024Article
- Article
- Warm Cells, Hot Mitochondria: Achievements and Problems of Ultralocal Thermometry.International journal of molecular sciences · 2023Review
- Photothermal Effects of High-Energy Photobiomodulation Therapies: An In Vitro Investigation.Biomedicines · 2023Article
- Vast heterogeneity in cytoplasmic diffusion rates revealed by nanorheology and Doppelgänger simulations.Biophysical journal · 2023Article
- Measurement of Intracellular Temperature in Brown Adipocytes Using a Cationic Fluorescent Polymeric Thermometer.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Heat Release by Isolated Mouse Brain Mitochondria Detected with Diamond Thermometer.Nanomaterials (Basel, Switzerland) · 2022Article
- Polymeric Nanoparticles with Embedded Eu(III) Complexes as Molecular Probes for Temperature Sensing.Molecules (Basel, Switzerland) · 2022Article
- Spatiotemporally mapping temperature dynamics of lysosomes and mitochondria using cascade organelle-targeting upconversion nanoparticles.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- A Ratiometric Organic Fluorescent Nanogel Thermometer for Highly Sensitive Temperature Sensing.Biosensors · 2022Article
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Authors and funding
4 authors.
Funding
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Abstract
Changes in intracellular temperatures reflect the activity of the cell. Thus, the tool to measure intracellular temperatures could provide valuable information about cellular status. We previously reported a method to analyze the intracellular temperature distribution using a fluorescent polymeric thermometer (FPT) in combination with fluorescence lifetime imaging microscopy (FLIM). Intracellular delivery of the FPT used in the previous study required microinjection. We now report a novel FPT that is cell permeable and highly photostable, and we describe the application of this FPT to the imaging of intracellular temperature distributions in various types of mammalian cell lines. This cell-permeable FPT displayed a temperature resolution of 0.05°C to 0.54°C within the range from 28°C to 38°C in HeLa cell extracts. Using our optimized protocol, this cell-permeable FPT spontaneously diffused into HeLa cells within 10 min of incubation and exhibited minimal toxicity over several hours of observation. FLIM analysis confirmed a temperature difference between the nucleus and the cytoplasm and heat production near the mitochondria, which were also detected previously using the microinjected FPT. We also showed that this cell-permeable FPT protocol can be applied to other mammalian cell lines, COS7 and NIH/3T3 cells. Thus, this cell-permeable FPT represents a promising tool to study cellular states and functions with respect to temperature.
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