ArticlePloS one2015
A de novo transcriptome and valid reference genes for quantitative real-time PCR in Colaphellus bowringi.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 60 citations in OpenAlex.
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- MAPK Signaling Pathway Is Essential for Female Reproductive Regulation in the Cabbage Beetle,Cells · 2022Article
- Reference Genes for Expression Analyses by qRT-PCR inInsects · 2022Article
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- Identification and Validation of Reference Genes for Gene Expression Analysis inFrontiers in physiology · 2022Article
- Steroid hormone ecdysone deficiency stimulates preparation for photoperiodic reproductive diapause.PLoS genetics · 2021Article
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- Evaluation of reference genes for quantitative real-time PCR normalization in the scarab beetle Holotrichia oblita.PloS one · 2020Article
- Identification and Validation of Reference Genes for Quantitative Gene Expression Analysis inFrontiers in physiology · 2020Article
- Evaluation of reference genes and expression of key genes involved in the isoprenoid metabolic pathway of rice leaves after infection by the Southern rice black-streaked dwarf virus.Molecular biology reports · 2019Article
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- Selection of Reference Genes for Expression Analysis in Chinese Medicinal HerbFrontiers in pharmacology · 2019Article
- Selection of Reference Genes for the Normalization of RT-qPCR Data in Gene Expression Studies in Insects: A Systematic Review.Frontiers in physiology · 2018Article
- Fatty acid synthase 2 contributes to diapause preparation in a beetle by regulating lipid accumulation and stress tolerance genes expression.Scientific reports · 2017Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe cabbage beetle Colaphellus bowringi Baly is a serious insect pest of crucifers and undergoes reproductive diapause in soil. An understanding of the molecular mechanisms of diapause regulation, insecticide resistance, and other physiological processes is helpful for developing new management strategies for this beetle. However, the lack of genomic information and valid reference genes limits knowledge on the molecular bases of these physiological processes in this species.
resultsUsing Illumina sequencing, we obtained more than 57 million sequence reads derived from C. bowringi, which were assembled into 39,390 unique sequences. A Clusters of Orthologous Groups classification was obtained for 9,048 of these sequences, covering 25 categories, and 16,951 were assigned to 255 Kyoto Encyclopedia of Genes and Genomes pathways. Eleven candidate reference gene sequences from the transcriptome were then identified through reverse transcriptase polymerase chain reaction. Among these candidate genes, EF1α, ACT1, and RPL19 proved to be the most stable reference genes for different reverse transcriptase quantitative polymerase chain reaction experiments in C. bowringi. Conversely, aTUB and GAPDH were the least stable reference genes.
conclusionThe abundant putative C. bowringi transcript sequences reported enrich the genomic resources of this beetle. Importantly, the larger number of gene sequences and valid reference genes provide a valuable platform for future gene expression studies, especially with regard to exploring the molecular mechanisms of different physiological processes in this species.
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