ArticleBiophysical journal2015
In situ determination of structure and fluctuations of coexisting fluid membrane domains.
Article in Biophysical journal, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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38 citing papers in PubMed, 97 citations in OpenAlex.
- Inside the membrane: a closer look using elastic scattering techniques and friends.European biophysics journal : EBJ · 2026Review
- Nanodomain formation in lipid bilayers II: The influence of mixed-chain saturated lipids.Biochimica et biophysica acta. Biomembranes · 2026Article
- Five key challenges for the plasma membrane insertion of nicotinic acetylcholine receptors in cellular model systems.Molecular and cellular biochemistry · 2026Review
- Nanodomain formation in lipid bilayers II: The influence of mixed-chain saturated lipids.bioRxiv : the preprint server for biology · 2026Article
- Confined Water Organization in the Fluid Phase of Phospholipid Self-Assemblies: The Three-Water Layer Model.Sub-cellular biochemistry · 2026Review
- Cryo-EM images of phase-separated lipid bilayer vesicles analyzed with a machine-learning approach.Biophysical journal · 2024Article
- Cholesterol and Lipid Rafts in the Biogenesis of Amyloid-β Protein and Alzheimer's Disease.Annual review of biophysics · 2024Review
- Disruption of liquid/liquid phase separation in asymmetric GUVs prepared by hemifusion.bioRxiv : the preprint server for biology · 2024Article
- Small-angle scattering from flat bilayers containing correlated scattering length density inhomogeneities.Journal of applied crystallography · 2023Article
- On Fusogenicity of Positively Charged Phased-Separated Lipid Vesicles: Experiments and Computational Simulations.Biomolecules · 2023Article
- Quantitative Comparison against Experiments Reveals Imperfections in Force Fields' Descriptions of POPC-Cholesterol Interactions.Journal of chemical theory and computation · 2023Article
- Viscosity-Sensitive Membrane Dyes as Tools To Estimate the Crystalline Structure of Lipid Bilayers.Analytical chemistry · 2023Article
- Visualizing lipid membrane structure with cryo-EM: past, present, and future.Emerging topics in life sciences · 2023Review
- Lipid21: Complex Lipid Membrane Simulations with AMBER.Journal of chemical theory and computation · 2022Article
- The Two Faces of the Liquid Ordered Phase.The journal of physical chemistry letters · 2022Article
- Lysolipids regulate raft size distribution.Frontiers in molecular biosciences · 2022Article
- Scaling relationships for the elastic moduli and viscosity of mixed lipid membranes.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Direct imaging of liquid domains in membranes by cryo-electron tomography.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Direct label-free imaging of nanodomains in biomimetic and biological membranes by cryogenic electron microscopy.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Laterally Resolved Small-Angle Scattering Intensity from Lipid Bilayer Simulations: An Exact and a Limited-Range Treatment.Journal of chemical theory and computation · 2020Article
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5 authors at 3 institutions in 1 country.
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Abstract
Biophysical understanding of membrane domains requires accurate knowledge of their structural details and elasticity. We report on a global small angle x-ray scattering data analysis technique for coexisting liquid-ordered (Lo) and liquid-disordered (Ld) domains in fully hydrated multilamellar vesicles. This enabled their detailed analysis for differences in membrane thickness, area per lipid, hydrocarbon chain length, and bending fluctuation as demonstrated for two ternary mixtures (DOPC/DSPC/CHOL and DOPC/DPPC/CHOL) at different cholesterol concentrations. Lo domains were found to be ~10 Å thicker, and laterally up to 20 Å(2)/lipid more condensed than Ld domains. Their bending fluctuations were also reduced by ~65%. Increase of cholesterol concentration caused significant changes in structural properties of Ld, while its influence on Lo properties was marginal. We further observed that temperature-induced melting of Lo domains is associated with a diffusion of cholesterol to Ld domains and controlled by Lo/Ld thickness differences.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.