ArticleBiophysical journal2015
Three-dimensional balance of cortical tension and axial contractility enables fast amoeboid migration.
Article in Biophysical journal, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 51 citations in OpenAlex.
- A role for PaxB in regulating blebbing: experimental insights and theoretical perspectives from Dictyostelium discoideum.BMC molecular and cell biology · 2026Article
- Emergence of multiple collective motility modes in a physical model of cell chains.NPJ systems biology and applications · 2025Article
- Mapping mechanical stress in curved epithelia of designed size and shape.Nature communications · 2023Article
- Computational modelling of cell motility modes emerging from cell-matrix adhesion dynamics.PLoS computational biology · 2022Article
- Push or pull: how cytoskeletal crosstalk facilitates nuclear movement through 3D environments.Physical biology · 2022Review
- Article
- RASSF1C oncogene elicits amoeboid invasion, cancer stemness, and extracellular vesicle release via a SRC/Rho axis.The EMBO journal · 2021Article
- Elucidating the Biomechanics of Leukocyte Transendothelial Migration by Quantitative Imaging.Frontiers in cell and developmental biology · 2021Review
- Measuring mechanical stress in living tissues.Nature reviews. Physics · 2020Article
- Pressure sensing through Piezo channels controls whether cells migrate with blebs or pseudopods.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Extent of Cell Confinement in Microtracks Affects Speed and Results in Differential Matrix Strains.Biophysical journal · 2019Article
- Fabrication and Implementation of a Reference-Free Traction Force Microscopy Platform.Journal of visualized experiments : JoVE · 2019Article
- Three-Dimensional Monolayer Stress Microscopy.Biophysical journal · 2019Article
- Reference-Free Traction Force Microscopy Platform Fabricated via Two-Photon Laser Scanning Lithography Enables Facile Measurement of Cell-Generated Forces.ACS applied materials & interfaces · 2019Article
- Article
- Connecting G protein signaling to chemoattractant-mediated cell polarity and cytoskeletal reorganization.Small GTPases · 2018Review
- Analysis of the Precision, Robustness, and Speed of Elastic Resonator Interference Stress Microscopy.Biophysical journal · 2018Article
- Mechanoreciprocity in cell migration.Nature cell biology · 2018Review
- Long-term imaging of cellular forces with high precision by elastic resonator interference stress microscopy.Nature cell biology · 2017Article
- Mechanosensitive Adhesion Explains Stepping Motility in Amoeboid Cells.Biophysical journal · 2017Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Fast amoeboid migration requires cells to apply mechanical forces on their surroundings via transient adhesions. However, the role these forces play in controlling cell migration speed remains largely unknown. We used three-dimensional force microscopy to measure the three-dimensional forces exerted by chemotaxing Dictyostelium cells, and examined wild-type cells as well as mutants with defects in contractility, internal F-actin crosslinking, and cortical integrity. We showed that cells pull on their substrate adhesions using two distinct, yet interconnected mechanisms: axial actomyosin contractility and cortical tension. We found that the migration speed increases when axial contractility overcomes cortical tension to produce the cell shape changes needed for locomotion. We demonstrated that the three-dimensional pulling forces generated by both mechanisms are internally balanced by an increase in cytoplasmic pressure that allows cells to push on their substrate without adhering to it, and which may be relevant for amoeboid migration in complex three-dimensional environments.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.