ArticleBioMed research international2015
Development of a loop-mediated isothermal amplification assay for rapid detection of Trichosporon asahii in experimental and clinical samples.
Article in BioMed research international, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed, 12 citations in OpenAlex.
- Establishment and Application of a Multiplex PCR Assay for Detection of Sclerotium rolfsii, Lasiodiplodia theobromae, and Fusarium oxysporum in Peanut.Molecular biotechnology · 2023Article
- Development of loop-mediated isothermal amplification for rapid detection of sporotrichosis caused byVeterinary world · 2023Article
- Recent advances in loop-mediated isothermal amplification (LAMP) for rapid and efficient detection of pathogens.Current research in microbial sciences · 2022Review
- Microfluidic Chip for Detection of Fungal Infections.ACS omega · 2019Article
- Rapid and Simple Detection ofBioMed research international · 2019Article
- Epidemiology and Outcome of Trichosporon Fungemia: A Review of 185 Reported Cases From 1975 to 2014.Open forum infectious diseases · 2015Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Invasive trichosporonosis is a deep mycosis found mainly in immunocompromised hosts, and the major pathogen is Trichosporon asahii. We detected the species-specific intergenic spacers (IGS) of rRNA gene of T. asahii using a loop-mediated isothermal amplification (LAMP) assay in 15 isolates with 3 different visualization methods, including SYBR green detection, gel electrophoresis, and turbidimetric methods. The LAMP assay displayed superior rapidity to other traditional methods in the detection time; that is, only 1 h was needed for detection and identification of the pathogen DNA. Furthermore, the detection limit of the LAMP assay was more sensitive than the PCR assay. We also successfully detect the presence of T. asahii in samples from experimentally infected mice and samples from patients with invasive trichosporonosis caused by T. asahii, suggesting that this method may become useful in clinical applications in the near future.
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