ArticleFrontiers in cellular and infection microbiology2015
Commensal E. coli Stx2 lysogens produce high levels of phages after spontaneous prophage induction.
Article in Frontiers in cellular and infection microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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19 citing papers in PubMed, 39 citations in OpenAlex.
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- Transcriptomic and proteomic analysis of the virulence inducing effect of ciprofloxacin on enterohemorrhagic Escherichia coli.PloS one · 2024Article
- Antimicrobial growth promoters approved in food-producing animals in South Africa induce shiga toxin-converting bacteriophages from Escherichia coli O157:H7.Gut pathogens · 2023Article
- CommensalAnimals : an open access journal from MDPI · 2023Article
- Characterization of a New TemperatePathogens (Basel, Switzerland) · 2022Article
- EnterohemorrhagicInternational journal of molecular sciences · 2022Review
- Pathogenomes and variations in Shiga toxin production among geographically distinct clones ofMicrobial genomics · 2022Article
- Replication Region Analysis Reveals Non-lambdoid Shiga Toxin Converting Bacteriophages.Frontiers in microbiology · 2021Article
- Vitamin K Analogs Influence the Growth and Virulence Potential of Enterohemorrhagic Escherichia coli.Applied and environmental microbiology · 2020Article
- Efficiency of induction of Shiga-toxin lambdoid prophages in Escherichia coli due to oxidative and antibiotic stress depends on the combination of prophage and the bacterial strain.Journal of applied genetics · 2020Article
- Carriage of Shiga toxin phage profoundly affects Escherichia coli gene expression and carbon source utilization.BMC genomics · 2019Article
- K5 Capsule and Lipopolysaccharide Are Important in Resistance to T4 Phage Attack in ProbioticFrontiers in microbiology · 2019Article
- Bacterial 'Grounded' Prophages: Hotspots for Genetic Renovation and Innovation.Frontiers in genetics · 2019Article
- Prophage induction, but not production of phage particles, is required for lethal disease in a microbiome-replete murine model of enterohemorrhagic E. coli infection.PLoS pathogens · 2019Article
- Closed Genome Sequence of Escherichia coli K-12 Group Strain C600.Microbiology resource announcements · 2019Article
- Fighting Fire with Fire: Phage Potential for the Treatment ofAntibiotics (Basel, Switzerland) · 2018Article
- Non-pathogenicFrontiers in microbiology · 2018Article
- Induction of Shiga Toxin-Encoding Prophage by Abiotic Environmental Stress in Food.Applied and environmental microbiology · 2017Article
- Article
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5 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Enterohemorrhagic E. coli (EHEC) is a food-borne pathogen that causes disease ranging from uncomplicated diarrhea to life-threatening hemolytic uremic syndrome (HUS) and nervous system complications. Shiga toxin 2 (Stx2) is the major virulence factor of EHEC and is critical for development of HUS. The genes encoding Stx2 are carried by lambdoid bacteriophages and the toxin production is tightly linked to the production of phages during lytic cycle. It has previously been suggested that commensal E. coli could amplify the production of Stx2-phages and contribute to the severity of disease. In this study we examined the susceptibility of commensal E. coli strains to the Stx2-converting phage ϕ734, isolated from a highly virulent EHEC O103:H25 (NIPH-11060424). Among 38 commensal E. coli strains from healthy children below 5 years, 15 were lysogenized by the ϕ734 phage, whereas lytic infection was not observed. Three of the commensal E. coli ϕ734 lysogens were tested for stability, and appeared stable and retained the phage for at least 10 cultural passages. When induced to enter lytic cycle by H2O2 treatment, 8 out of 13 commensal lysogens produced more ϕ734 phages than NIPH-11060424. Strikingly, five of them even spontaneously (non-induced) produced higher levels of phage than the H2O2 induced NIPH-11060424. An especially high frequency of HUS (60%) was seen among children infected by NIPH-11060424 during the outbreak in 2006. Based on our findings, a high Stx2 production by commensal E. coli lysogens cannot be ruled out as a contributor to the high frequency of HUS during this outbreak.
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