ArticleThe Plant cell2015
Genome-wide characterization of cis-acting DNA targets reveals the transcriptional regulatory framework of opaque2 in maize.
Article in The Plant cell, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 95 papers.
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Who cites it
95 citing papers in PubMed.
- The ZmmiR169o/ZmNF-YA13 module orchestrates maize kernel texture by fine-tuning starch biosynthesis and endosperm architecture.BMC plant biology · 2026Article
- The OsMYB4 transcription factor enables coordinated improvement of grain yield and quality in rice.Nature communications · 2026Article
- Subclass III SnRK2 Kinases Coordinate Starch and Storage Protein Synthesis During Maize Kernel Development.Plant biotechnology journal · 2026Article
- ZmMYB127 Modulates Maize Kernel Texture and Size by Integrating the Synthesis of Starch, Zein Proteins and Auxin.Plant biotechnology journal · 2026Article
- Transactivation of AGPL2 by Opaque2 is responsible for starch accumulation and kernel hardness in sweet corn.Journal of experimental botany · 2026Article
- Genome wide identification and analysis of inositol tetrakisphosphate kinase (ITPK) gene family in Triticum aestivum L.PloS one · 2026Article
- Decades' progress and prospects on maize functional genomics and molecular breeding.Science China. Life sciences · 2025Review
- TaSED interacts with TaSPA synergistically regulating SDS-sedimentation volume in bread wheat.Journal of integrative plant biology · 2025Article
- Designing a nitrogen-efficient cold-tolerant maize for modern agricultural systems.The Plant cell · 2025Review
- Identification of HSSP1 as a regulator of soybean protein content through QTL analysis and Soy-SPCC network.Plant biotechnology journal · 2025Article
- Transcriptome profiling of maize transcription factor mutants to probe gene regulatory network predictions.G3 (Bethesda, Md.) · 2025Article
- E3 ligase SlCOP1-1 stabilizes transcription factor SlOpaque2 and enhances fruit resistance to Botrytis cinerea in tomato.Plant physiology · 2024Article
- The transcription factor CAMTA2 interacts with the histone acetyltransferase GCN5 and regulates grain weight in wheat.The Plant cell · 2024Article
- Article
- Central Roles of ZmNAC128 and ZmNAC130 in Nutrient Uptake and Storage during Maize Grain Filling.Genes · 2024Article
- Editing the 19 kDa alpha-zein gene family generates non-opaque2-based quality protein maize.Plant biotechnology journal · 2024Article
- Decoding the gene regulatory network of endosperm differentiation in maize.Nature communications · 2024Article
- Multi-layer molecular analysis reveals distinctive metabolomic and transcriptomic profiles of different sweet corn varieties.Frontiers in plant science · 2024Article
- NAKED ENDOSPERM1, NAKED ENDOSPERM2, and OPAQUE2 interact to regulate gene networks in maize endosperm development.The Plant cell · 2023Article
- The transcription factors ZmNAC128 and ZmNAC130 coordinate with Opaque2 to promote endosperm filling in maize.The Plant cell · 2023Article
35 more citing papers are in PubMed but not listed here.
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12 authors.
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Abstract
Opaque2 (O2) is a transcription factor that plays important roles during maize endosperm development. Mutation of the O2 gene improves the nutritional value of maize seeds but also confers pleiotropic effects that result in reduced agronomic quality. To reveal the transcriptional regulatory framework of O2, we studied the transcriptome of o2 mutants using RNA sequencing (RNA-Seq) and determined O2 DNA binding targets using chromatin immunoprecipitation coupled to high-throughput sequencing (ChIP-Seq). The RNA-Seq analysis revealed 1605 differentially expressed genes (DEGs) and 383 differentially expressed long, noncoding RNAs. The DEGs cover a wide range of functions related to nutrient reservoir activity, nitrogen metabolism, stress resistance, etc. ChIP-Seq analysis detected 1686 O2 DNA binding sites distributed over 1143 genes. Overlay of the RNA-Seq and ChIP-Seq results revealed 35 O2-modulated target genes. We identified four O2 binding motifs; among them, TGACGTGG appears to be the most conserved and strongest. We confirmed that, except for the 16- and 18-kD zeins, O2 directly regulates expression of all other zeins. O2 directly regulates two transcription factors, genes linked to carbon and amino acid metabolism and abiotic stress resistance. We built a hierarchical regulatory model for O2 that provides an understanding of its pleiotropic biological effects.
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