ArticleMolecular and cellular biology2015
Characterization of BRD4 during mammalian postmeiotic sperm development.
Article in Molecular and cellular biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
23 citing papers in PubMed, 44 citations in OpenAlex.
- Chromatin environment-dependent effects of DOT1L on gene expression in male germ cells.Communications biology · 2025Article
- RAD51 and Infertility: A Review and Case-Control Study.Biochemical genetics · 2024Review
- Contribution of the paternal histone epigenome to the preimplantation embryo.Frontiers in cell and developmental biology · 2024Review
- The DOT1L-MLLT10 complex regulates male fertility and promotes histone removal during spermiogenesis.Development (Cambridge, England) · 2023Article
- A shared 'vulnerability code' underpins varying sources of DNA damage throughout paternal germline transmission in mouse.Nucleic acids research · 2023Article
- BET bromodomain inhibitor JQ1 regulates spermatid development by changing chromatin conformation in mouse spermatogenesis.Genes & diseases · 2022Article
- BET Proteins Regulate Expression of Osr1 in Early Kidney Development.Biomedicines · 2021Article
- Article
- Testis-expressed protein 33 is not essential for spermiogenesis and fertility in mice.Molecular medicine reports · 2021Article
- Genome-wide occupancy reveals the localization of H1T2 (H1fnt) to repeat regions and a subset of transcriptionally active chromatin domains in rat spermatids.Epigenetics & chromatin · 2021Article
- Battle of the Sex Chromosomes: Competition between X and Y Chromosome-Encoded Proteins for Partner Interaction and Chromatin Occupancy Drives Multicopy Gene Expression and Evolution in Muroid Rodents.Molecular biology and evolution · 2020Article
- Super-enhancer switching drives a burst in gene expression at the mitosis-to-meiosis transition.Nature structural & molecular biology · 2020Article
- Article
- Multi-omic analysis of gametogenesis reveals a novel signature at the promoters and distal enhancers of active genes.Nucleic acids research · 2020Article
- Gcn5-Mediated Histone Acetylation Governs Nucleosome Dynamics in Spermiogenesis.Developmental cell · 2019Article
- Testis specific histone 2B is associated with sperm chromatin dynamics and bull fertility-a pilot study.Reproductive biology and endocrinology : RB&E · 2017Article
- SLY regulates genes involved in chromatin remodeling and interacts with TBL1XR1 during sperm differentiation.Cell death and differentiation · 2017Article
- tBRD-1 and tBRD-2 regulate expression of genes necessary for spermatid differentiation.Biology open · 2017Article
- Testis-specific transcriptional regulators selectively occupy BORIS-bound CTCF target regions in mouse male germ cells.Scientific reports · 2017Article
- The Role of Bromodomain Testis-Specific Factor, BRDT, in Cancer: A Biomarker and A Possible Therapeutic Target.Cell journal · 2017Review
Corrections and comments
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Authors and funding
11 authors at 2 institutions in 1 country.
Funding
Abstract
During spermiogenesis, the postmeiotic phase of mammalian spermatogenesis, transcription is progressively repressed as nuclei of haploid spermatids are compacted through a dramatic chromatin reorganization involving hyperacetylation and replacement of most histones with protamines. Although BRDT functions in transcription and histone removal in spermatids, it is unknown whether other BET family proteins play a role. Immunofluorescence of spermatogenic cells revealed BRD4 in a ring around the nuclei of spermatids containing hyperacetylated histones. The ring lies directly adjacent to the acroplaxome, the cytoskeletal base of the acrosome, previously linked to chromatin reorganization. The BRD4 ring does not form in acrosomal mutant mice. Chromatin immunoprecipitation followed by sequencing in spermatids revealed enrichment of BRD4 and acetylated histones at the promoters of active genes. BRD4 and BRDT show distinct and synergistic binding patterns, with a pronounced enrichment of BRD4 at spermatogenesis-specific genes. Direct association of BRD4 with acetylated H4 decreases in late spermatids as acetylated histones are removed from the condensing nucleus in a wave following the progressing acrosome. These data provide evidence of a prominent transcriptional role for BRD4 and suggest a possible removal mechanism for chromatin components from the genome via the progressing acrosome as transcription is repressed and chromatin is compacted during spermiogenesis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.