ArticlemBio2015
Chromosome organization and replisome dynamics in Mycobacterium smegmatis.
Article in mBio, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 73 citations in OpenAlex.
- Chromosome segregation dynamics during the cell cycle of Staphylococcus aureus.Nature communications · 2025Article
- Nonequilibrium polysome dynamics promote chromosome segregation and its coupling to cell growth ineLife · 2025Article
- Archaeal SegAB forms a bipolar structure that promotes chromosome segregation in spherical cells.bioRxiv : the preprint server for biology · 2025Article
- Nonequilibrium polysome dynamics promote chromosome segregation and its coupling to cell growth inbioRxiv : the preprint server for biology · 2025Article
- MksB is a novel mycobacterial condensin that orchestrates spatiotemporal positioning of replication machinery.Scientific reports · 2024Article
- Chromosome organization shapes replisome dynamics in Caulobacter crescentus.Nature communications · 2024Article
- Investigating the composition and recruitment of the mycobacterial ImuA'-ImuB-DnaE2 mutasome.eLife · 2023Article
- Septum site placement inMicrobiology (Reading, England) · 2023Article
- The Quantification of Bacterial Cell Size: Discrepancies Arise from Varied Quantification Methods.Life (Basel, Switzerland) · 2023Article
- Article
- Drug resistant tuberculosis: Implications for transmission, diagnosis, and disease management.Frontiers in cellular and infection microbiology · 2022Review
- Catching a Walker in the Act-DNA Partitioning by ParA Family of Proteins.Frontiers in microbiology · 2022Review
- Spatiotemporal localization of proteins in mycobacteria.Cell reports · 2021Article
- Lsr2, a nucleoid-associated protein influencing mycobacterial cell cycle.Scientific reports · 2021Article
- ClpX Is Essential and Activated by Single-Strand DNA Binding Protein in Mycobacteria.Journal of bacteriology · 2021Article
- Single-Cell Analysis of Mycobacteria Using Microfluidics and Time-Lapse Microscopy.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Exploiting Fluorescent Proteins to Understand Mycobacterium tuberculosis Biology.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Article
- Cell-Cycle-Associated Expression Patterns Predict Gene Function in Mycobacteria.Current biology : CB · 2020Article
- Bacterial chromosome segregation by the ParABS system.Open biology · 2020Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
unlabelledSubcellular organization of the bacterial nucleoid and spatiotemporal dynamics of DNA replication and segregation have been studied intensively, but the functional link between these processes remains poorly understood. Here we use quantitative time-lapse fluorescence microscopy for single-cell analysis of chromosome organization and DNA replisome dynamics in Mycobacterium smegmatis. We report that DNA replication takes place near midcell, where, following assembly of the replisome on the replication origin, the left and right replication forks colocalize throughout the replication cycle. From its initial position near the cell pole, a fluorescently tagged chromosomal locus (attB, 245° from the origin) moves rapidly to the replisome complex just before it is replicated. The newly duplicated attB loci then segregate to mirror-symmetric positions relative to midcell. Genetic ablation of ParB, a component of the ParABS chromosome segregation system, causes marked defects in chromosome organization, condensation, and segregation. ParB deficiency also results in mislocalization of the DNA replication machinery and SMC (structural maintenance of chromosome) protein. These observations suggest that ParB and SMC play important and overlapping roles in chromosome organization and replisome dynamics in mycobacteria. IMPORTANCE: We studied the spatiotemporal organization of the chromosome and DNA replication machinery in Mycobacterium smegmatis, a fast-growing relative of the human pathogen Mycobacterium tuberculosis. We show that genetic ablation of the DNA-binding proteins ParB and SMC disturbs the organization of the chromosome and causes a severe defect in subcellular localization and movement of the DNA replication complexes. These observations suggest that ParB and SMC provide a functional link between chromosome organization and DNA replication dynamics. This work also reveals important differences in the biological roles of the ParABS and SMC systems in mycobacteria versus better-characterized model organisms, such as Escherichia coli and Bacillus subtilis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.