ArticleApplied microbiology and biotechnology2015
The feasibility of using irreversible electroporation to introduce pores in bacterial cellulose scaffolds for tissue engineering.
Article in Applied microbiology and biotechnology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 22 citations in OpenAlex.
- Efficient Decellularization by Application of Moderate High Hydrostatic Pressure with Supercooling Pretreatment.Micromachines · 2021Article
- High-Voltage, Pulsed Electric Fields EliminateAdvances in wound care · 2021Article
- Scaffold-Based Tissue Engineering Strategies for Osteochondral Repair.Frontiers in bioengineering and biotechnology · 2021Review
- Structural changes of bacterial nanocellulose pellicles induced by genetic modification of Komagataeibacter hansenii ATCC 23769.Applied microbiology and biotechnology · 2019Article
- Scaffolds for Chondrogenic Cells Cultivation Prepared from Bacterial Cellulose with Relaxed Fibers Structure Induced Genetically.Nanomaterials (Basel, Switzerland) · 2018Article
- Non-invasive nanosecond electroporation for biocontrol of surface infections: an in vivo study.Scientific reports · 2018Article
- Synergistic combinations of short high-voltage pulses and long low-voltage pulses enhance irreversible electroporation efficacy.Scientific reports · 2017Article
- Metabolic Investigation in Gluconacetobacter xylinus and Its Bacterial Cellulose Production under a Direct Current Electric Field.Frontiers in microbiology · 2016Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
Abstract
This work investigates the feasibility of the use of irreversible electroporation (IRE) in the biofabrication of 3D cellulose nanofibril networks via the bacterial strain Gluconacetobacter xylinus. IRE uses electrical pulses to increase membrane permeability by altering the transmembrane potential; past a threshold, damage to the cell becomes too great and leads to cell death. We hypothesized that using IRE to kill the bacteria at specific locations and particular times, we could introduce conduits in the overall scaffold by preventing cellulose biosynthesis locally. Through mathematical modeling and experimental techniques, electrical effects were investigated and the parameters for IRE of G. xylinus were determined. We found that for a specific set of parameters, an applied electric field of 8 to 12.5 kV/cm, producing a local field of 3 kV/cm, was sufficient to kill most of the bacteria and create a localized pore. However, an applied electric field of 17.5 kV/cm was required to kill all. Results suggest that IRE may be an effective tool to create scaffolds with appropriate porosity for orthopedic applications. Ideally, these engineered scaffolds could be used to successfully treat osteochondral defects.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.