Trial reportPloS one2015
Antiplaque effect of essential oils and 0.2% chlorhexidine on an in situ model of oral biofilm growth: a randomised clinical trial.
Trial report in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to 3 registered trials, which are not on this map. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Antiplaque Effect of Essential Oils and 0.2% Chlorhexidine on an in Situ Model of Oral Biofilm Growth: a Randomised Clinical Trial.
Antiplaque Effect of Essential Oils and 0.2% Chlorhexidine on an in Situ Model of Oral Biofilm Growth. A Randomized Clinical Trial
Essential Oils With and Without Alcohol: in Vivo Antibacterial Effect
Who cites it
16 citing papers in PubMed, 41 citations in OpenAlex.
- Comparison of Clinical Effects of Essential Oil-Containing Mouthwash and Chlorhexidine Mouth Wash in Patients Undergoing Fixed Orthodontic Treatment - Three-Month Follow-up Randomized-Controlled Trial.Clinical and experimental dental research · 2026Trial
- Randomised-crossover clinical trial on the substantivity of a single application of a gel containing chlorhexidine and o-cymen-5-ol on the oral biofilm and saliva.BMC oral health · 2024Trial
- Comparative Evaluation of Antimicrobial Activity of Dentin Disinfection Protocols Against MRSA andJournal of functional biomaterials · 2026Article
- The effect of fruit extracts containing phenolic compounds on the survivability of Streptococcus mutans: an in vitro study.European archives of paediatric dentistry : official journal of the European Academy of Paediatric Dentistry · 2026Article
- Host-microbiome-immune disequilibrium in oral disease: mechanisms, dysbiosis, and precision therapeutics.Frontiers in immunology · 2026Review
- In Vitro Assessment of Salivary Pellicle Disruption and Biofilm Removal on Titanium: Exploring the Role of Surface Hydrophobicity in Chemical Disinfection.Clinical and experimental dental research · 2025Article
- The Effect of Coconut and Frankincense Oils on the Biofilm Growth ofF1000Research · 2025Article
- Effect of Plant-Based Mouthwash (Medicina (Kaunas, Lithuania) · 2023Article
- Short-term effects of Chlorhexidine mouthwash and Listerine on oral microbiome in hospitalized patients.Frontiers in cellular and infection microbiology · 2023Article
- Pneumonia prevention in the elderly patients: the other sides.Aging clinical and experimental research · 2021Article
- Effects of Cinnamon (Molecules (Basel, Switzerland) · 2020Review
- Intraoral appliances for in situ oral biofilm growth: a systematic review.Journal of oral microbiology · 2019Review
- Article
- Article
- Anti-biofilm Activity as a Health Issue.Frontiers in microbiology · 2016Review
- Devices for In situ Development of Non-disturbed Oral Biofilm. A Systematic Review.Frontiers in microbiology · 2016Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveTo evaluate the in situ antiplaque effect after 4 days of using of 2 commercial antimicrobial agents in short term on undisturbed plaque-like biofilm. TRIAL DESIGN AND
participantsAn observer-masked, crossover randomised clinical trial on 15 oral and systemically healthy volunteers between 20-30 years who were randomly and sequentially allocated in the same group which performed 3 interventions in different randomised sequences.
interventionThe participants wore an appliance in 3 different rinsing periods doing mouthwashes twice a day (1/0/1) with essential oils, 0.2% chlorhexidine or sterile water (negative control). At the end of each 4-day mouthwash period, samples were removed from the appliance. Posteriorly, after bacterial vital staining, samples were analysed using a Confocal Laser Scanning Microscope.
main outcome measuresBacterial vitality, thickness and covering grade by the biofilm after 4 days of applying each of the mouthwashes.
resultsThe essential oils and the 0.2% chlorhexidine were significantly more effective than the sterile water at reducing bacterial vitality, thickness and covering grade by the biofilm. No significant differences were found between the 0.2% chlorhexidine and the essential oils at reducing the bacterial vitality (13.2% vs. 14.7%). However, the 0.2% chlorhexidine showed more reduction than the essential oils in thickness (6.5 μm vs. 10.0 μm; p<0.05) and covering grade by the biofilm (20.0% vs. 54.3%; p<0.001).
conclusionThe essential oils and 0.2% chlorhexidine showed a high antiplaque effect. Although the 0.2% chlorhexidine showed better results with regard to reducing the thickness and covering grade by the biofilm, both antiseptics showed a high and similar antibacterial activity. CLINICAL RELEVANCE: Daily essential oils or 0.2% chlorhexidine mouthwashes are effective when reducing dental plaque formation in the short term. Although 0.2% chlorhexidine continues to be the "gold standard" in terms of antiplaque effect, essential oils could be considered a reliable alternative.
trial registrationClinicalTrials.gov NCT02124655.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.