ArticlePloS one2015
Rapid identification of medically important Candida isolates using high resolution melting analysis.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.
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Who cites it
20 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Nucleic Acid Amplification Tests forPathogens (Basel, Switzerland) · 2026Pooled it
- Peering Inside the Black Box: Explainable AI to Interpret Advanced Computer Vision Fungal Pathogen Prediction.Scientific reports · 2026Article
- Integrating Clinical and Microbiological Expertise to Improve Vaginal Candidiasis Management.Mycopathologia · 2024Article
- Multiplex size marker (YEAST PLEX) for rapid and accurate identification of pathogenic yeasts.Journal of clinical laboratory analysis · 2022Article
- Molecular Diagnosis of Yeast Infections.Current fungal infection reports · 2021Review
- Molecular identification of Candida isolates by Real-time PCR-high-resolution melting analysis and investigation of the genetic diversity of Candida species.Journal of clinical laboratory analysis · 2020Article
- Rapid identification of the Candida glabrata species complex by high-resolution melting curve analysis.Journal of clinical laboratory analysis · 2020Article
- Article
- Evaluation of a Novel Mitochondrial Pan-Journal of fungi (Basel, Switzerland) · 2019Article
- Recent trends in molecular diagnostics of yeast infections: from PCR to NGS.FEMS microbiology reviews · 2019Review
- Capability of Fluorescent Capillary Electrophoresis To Distinguish Species of theJournal of clinical microbiology · 2019Article
- Fluorescent Capillary Electrophoresis Is Superior to Culture in DetectingJournal of clinical microbiology · 2019Article
- The Impact of DNA Extraction Methods on Stool Bacterial and Fungal Microbiota Community Recovery.Frontiers in microbiology · 2019Article
- Validation of a simplex PCR assay enabling reliable identification of clinically relevant Candida species.BMC infectious diseases · 2018Article
- Assessment of Multi Fragment Melting Analysis System (MFMAS) for the Identification of Food-Borne Yeasts.Current microbiology · 2018Article
- Fast and Reliable Differentiation of Eight Trichinella Species Using a High Resolution Melting Assay.Scientific reports · 2017Article
- Discrimination of three genetically close Aspergillus species by using high resolution melting analysis applied to indoor air as case study.BMC microbiology · 2017Article
- Dynamic time warping assessment of high-resolution melt curves provides a robust metric for fungal identification.PloS one · 2017Article
- Evaluation of Fluorescent Capillary Electrophoresis for Rapid Identification of Candida Fungal Infections.Journal of clinical microbiology · 2016Article
- An update on current and novel molecular diagnostics for the diagnosis of invasive fungal infections.Expert review of molecular diagnosticsReview
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An increasing trend in non albicans infections and various susceptibility patterns to antifungal agents implies a requirement for the quick and reliable identification of a number of medically important Candida species. Real-time PCR followed by high resolution melting analysis (HRMA) was developed, tested on 25 reference Candida collection strains and validated on an additional 143 clinical isolates in this study. All reference strains and clinical isolates inconclusive when using phenotypic methods and/or HRMA were analysed using ITS2 sequencing. Considering reference and clinical strains together, 23 out of 27 Candida species could be clearly distinguished by HRMA, while the remaining 4 species were grouped in 2 pairs, when applying the mean Tm ± 3 SD values, the shape of the derivative melting curve (dMelt curve) and, in some cases, the normalized and temperature-shifted difference plot against C. krusei. HRMA as a simple, rapid and inexpensive tool was shown to be useful in identifying a wide spectrum of clinically important Candida species. It may complement the current clinical diagnostic approach based on commercially available biochemical kits.
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