ArticlePlant biotechnology journal2016
Genome-wide survey of artificial mutations induced by ethyl methanesulfonate and gamma rays in tomato.
Article in Plant biotechnology journal, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 73 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
73 citing papers in PubMed, 163 citations in OpenAlex.
- Ethyl methanesulfonate mutagenesis in fungi: genetic mechanisms, applications, and implications for agricultural biotechnology.Archives of microbiology · 2026Review
- Comparative Transcriptomic Analysis Reveals Divergent Stress Adaptation Strategies in Gamma-Induced Soybean Mutants.Plants (Basel, Switzerland) · 2026Article
- EMS-induced genomic variation and QTL-seq analysis of safflower spininess through whole genome sequencing (WGS).BMC genomics · 2025Article
- The impact of carbon-ion beam irradiation on the phenotypic and molecular variation of wheat.BMC plant biology · 2025Article
- Review
- Progress and innovations of gene cloning in wheat and its close relatives.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Review
- Genetic Diversity Analysis and Comprehensive Evaluation of "M82" in EMS-Mutagenized Tomato.Genes · 2025Article
- Effects of acute and chronic gamma irradiations on regeneration of tissue types from selected Ugandan cassava genotypes.Frontiers in plant science · 2025Article
- Development of Ac- and Ds-tagged starter lines for large-scale transposon-mutagenesis in tomato.PloS one · 2025Article
- Near-complete genome assembly of tomato (Plant biotechnology (Tokyo, Japan) · 2024Article
- Genotyping by sequencing; a strategy for identification and mapping of induced mutation in newly developed wheat mutant lines.Functional & integrative genomics · 2024Article
- Genomic variation across distribution of Micro-Tom, a model cultivar of tomato (Solanum lycopersicum).DNA research : an international journal for rapid publication of reports on genes and genomes · 2024Article
- Propagation path of a flowering cherry (Cerasus × yedoensis) cultivar 'Somei-Yoshino' traced by somatic mutations.DNA research : an international journal for rapid publication of reports on genes and genomes · 2024Article
- Furfural tolerance of mutant Saccharomyces cerevisiae selected via ionizing radiation combined with adaptive laboratory evolution.Biotechnology for biofuels and bioproducts · 2024Article
- Genomic variation induced by a low concentration of ethyl methanesulfonate (EMS) in quinoa 'Longli-4' variety.Botanical studies · 2024Article
- The transcription factor FcMYB3 responds toFrontiers in plant science · 2024Article
- Comparative Analysis of Physical and Chemical Mutagenesis in Chrysanthemum cv. 'Candid': Assessing Genetic Variation and Breeding Potential.ACS omega · 2023Article
- A large-scale whole-exome sequencing mutant resource for functional genomics in wheat.Plant biotechnology journal · 2023Article
- Article
- Augmenting tomato functional genomics with a genome-wide induced genetic variation resource.Frontiers in plant science · 2023Article
13 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Genome-wide mutations induced by ethyl methanesulfonate (EMS) and gamma irradiation in the tomato Micro-Tom genome were identified by a whole-genome shotgun sequencing analysis to estimate the spectrum and distribution of whole-genome DNA mutations and the frequency of deleterious mutations. A total of ~370 Gb of paired-end reads for four EMS-induced mutants and three gamma-ray-irradiated lines as well as a wild-type line were obtained by next-generation sequencing technology. Using bioinformatics analyses, we identified 5920 induced single nucleotide variations and insertion/deletion (indel) mutations. The predominant mutations in the EMS mutants were C/G to T/A transitions, while in the gamma-ray mutants, C/G to T/A transitions, A/T to T/A transversions, A/T to G/C transitions and deletion mutations were equally common. Biases in the base composition flanking mutations differed between the mutagenesis types. Regarding the effects of the mutations on gene function, >90% of the mutations were located in intergenic regions, and only 0.2% were deleterious. In addition, we detected 1,140,687 spontaneous single nucleotide polymorphisms and indel polymorphisms in wild-type Micro-Tom lines. We also found copy number variation, deletions and insertions of chromosomal segments in both the mutant and wild-type lines. The results provide helpful information not only for mutation research, but also for mutant screening methodology with reverse-genetic approaches.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.