ArticlePloS one2015
Antagonistic mechanism of iturin A and plipastatin A from Bacillus amyloliquefaciens S76-3 from wheat spikes against Fusarium graminearum.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 107 papers.
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Who cites it
107 citing papers in PubMed, 303 citations in OpenAlex.
- Bacillus velezensis EU07 suppresses Fusarium graminearum via transcriptomic reprogramming.Applied microbiology and biotechnology · 2026Article
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- Biogeography influences plant-microbe interactions and natural soil suppressiveness to black root rot disease of tobacco.Genome biology · 2025Article
- Unlocking olive rhizobacteria: harnessing biocontrol power to combat olive root rot and promote plant growth.International microbiology : the official journal of the Spanish Society for Microbiology · 2025Article
- 3-Methyl pentanoic acid suppress gray mold disease potentially targeting cell-wall integrity (CWI) and mitogen-activated protein kinase (MAPK) pathways in Botrytis cinerea.BMC microbiology · 2025Article
- Confrontations of the Pathogenic Fungus Colletotrichum graminicola With a Biocontrol Bacterium or a Ubiquitous Fungus Trigger Synthesis of Secondary Metabolites With Lead Structures of Synthetic Fungicides.Environmental microbiology · 2025Article
- Study on the Inhibitory Effects of Three EndophyticMetabolites · 2025Article
- Plipastatin is a shared good by Bacillus subtilis during combating Fusarium spp.FEMS microbiology ecology · 2025Article
- Efficacy of Selected Bacterial Strains in the Protection and Growth Stimulation of Winter Wheat and Maize.Plants (Basel, Switzerland) · 2025Article
- Antimicrobial Activity ofInternational journal of molecular sciences · 2025Review
- CFrontiers in microbiology · 2025Article
- Omics insights intoFrontiers in microbiology · 2025Article
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- Bacillus velezensis BE2 controls wheat and barley diseases by direct antagonism and induced systemic resistance.Applied microbiology and biotechnology · 2024Article
- Resistance Mechanisms of Plant Pathogenic Fungi to Fungicide, Environmental Impacts of Fungicides, and Sustainable Solutions.Plants (Basel, Switzerland) · 2024Review
- Enhanced low-cost lipopeptide biosurfactant production by Bacillus velezensis from residual glycerin.Bioprocess and biosystems engineering · 2024Article
- Seed Priming with RhizosphericToxins · 2024Article
- Inhibitory activity of bacterial lipopeptides against Fusarium oxysporum f.sp. Strigae.BMC microbiology · 2024Article
47 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Controlling toxigenic Fusarium graminearum (FG) is challenging. A bacterial strain (S76-3, identified as Bacillus amyloliquefaciens) that was isolated from diseased wheat spikes in the field displayed strong antifungal activity against FG. Reverse-phase high performance liquid chromatography and electrospray ionization mass spectrometry analyses revealed that S76-3 produced three classes of cyclic lipopeptides including iturin, plipastatin and surfactin. Each class consisted of several different molecules. The iturin and plipastatin fractions strongly inhibited FG; the surfactin fractions did not. The most abundant compound that had antagonistic activity from the iturin fraction was iturin A (m/z 1043.35); the most abundant active compound from the plipastatin fraction was plipastatin A (m/z 1463.90). These compounds were analyzed with collision-induced dissociation mass spectrometry. The two purified compounds displayed strong fungicidal activity, completely killing conidial spores at the minimal inhibitory concentration range of 50 µg/ml (iturin A) and 100 µg/ml (plipastatin A). Optical and fluorescence microscopy analyses revealed severe morphological changes in conidia and substantial distortions in FG hyphae treated with iturin A or plipastatin A. Iturin A caused leakage and/or inactivation of FG cellular contents and plipastatin A caused vacuolation. Time-lapse imaging of dynamic antagonistic processes illustrated that iturin A caused distortion and conglobation along hyphae and inhibited branch formation and growth, while plipastatin A caused conglobation in young hyphae and branch tips. Transmission electron microscopy analyses demonstrated that the cell walls of conidia and hyphae of iturin A and plipastatin A treated FG had large gaps and that their plasma membranes were severely damaged and separated from cell walls.
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