ArticleYeast (Chichester, England)2015
Puromycin- and methotrexate-resistance cassettes and optimized Cre-recombinase expression plasmids for use in yeast.
Article in Yeast (Chichester, England), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 19 citations in OpenAlex.
- A functional surface display system iniScience · 2025Article
- Enhanced metabolic entanglement emerges during the evolution of an interkingdom microbial community.Nature communications · 2024Article
- Recycling of cell surface membrane proteins from yeast endosomes is regulated by ubiquitinated Ist1.The Journal of cell biology · 2022Article
- Endosomal cargo recycling mediated by Gpa1 and phosphatidylinositol 3-kinase is inhibited by glucose starvation.Molecular biology of the cell · 2022Article
- Article
- The Rpd3-Complex Regulates Expression of Multiple Cell Surface Recycling Factors in Yeast.International journal of molecular sciences · 2021Article
- Treatment with surfactants enables quantification of translational activity by O-propargyl-puromycin labelling in yeast.BMC microbiology · 2021Article
- A glucose-starvation response governs endocytic trafficking and eisosomal retention of surface cargoes in budding yeast.Journal of cell science · 2021Article
- A probiotic yeast-based immunotherapy againstScience translational medicine · 2020Article
- Genetic dissection of early endosomal recycling highlights a TORC1-independent role for Rag GTPases.The Journal of cell biology · 2017Article
- Tetraspan cargo adaptors usher GPI-anchored proteins into multivesicular bodies.Cell cycle (Georgetown, Tex.) · 2015Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Here we expand the set of tools for genetically manipulating Saccharomyces cerevisiae. We show that puromycin-resistance can be achieved in yeast through expression of a bacterial puromycin-resistance gene optimized to the yeast codon bias, which in turn serves as an easy-to-use dominant genetic marker suitable for gene disruption. We have constructed a similar DNA cassette expressing yeast codon-optimized mutant human dihydrofolate reductase (DHFR), which confers resistance to methotrexate and can also be used as a dominant selectable marker. Both of these drug-resistant marker cassettes are flanked by loxP sites, allowing for their excision from the genome following expression of Cre-recombinase. Finally, we have created a series of plasmids for low-level constitutive expression of Cre-recombinase in yeast that allows for efficient excision of loxP-flanked markers.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.