ArticlePlant methods2015
A rapid and sensitive method for determination of carotenoids in plant tissues by high performance liquid chromatography.
Article in Plant methods, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers, 1 of them a synthesis that pooled it.
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Who cites it
58 citing papers in PubMed, 1 synthesis or guideline pooled it, 192 citations in OpenAlex.
- Pooled it
- Carotenoid production by Metschnikowia sp.: isolation from environmental samples and optimization of culture conditions.Biotechnology letters · 2026Article
- Dissecting the contributions to non-photochemical quenching in a land plant under fluctuating light.Nature communications · 2026Article
- Soil environment, physiological metabolism and ornamental traits of Zinnia response to water and nitrogen combination in a cold and arid environment.Frontiers in plant science · 2026Article
- First Report onAquaculture nutrition · 2026Article
- Effect of prolonged cooking on pro-vitamin A levels of biofortified East African highland bananas.GM crops & food · 2025Article
- Advances in biomanufacturing and recent biological applications of the fundamental bacterial carotenoids: a comprehensive review.Microbial cell factories · 2025Review
- Upregulation of Canthaxanthin Biosynthesis byBiology · 2025Article
- An Algal Nutrient-Replete, Optimized Medium for Fast Growth and High Triacylglycerol Accumulation.Plant direct · 2025Article
- Article
- Nutritional Enhancement of Crackers Through the Incorporation of By-Products from the Frozen Pumpkin Industry.Foods (Basel, Switzerland) · 2025Article
- Boosting Antioxidant Quality in Cucumber Beverages with Encapsulated Tomato Carotenoids.Antioxidants (Basel, Switzerland) · 2025Article
- Time-resolved oxidative signal convergence across the algae-embryophyte divide.Nature communications · 2025Article
- Plant chloroplast stress response: insights from mass spectrometry metabolites analysis.Frontiers in plant science · 2025Article
- Canned complementary porridges for infants and young children (6-23 months) based on African indigenous crops; nutritional content, consistency, sensory, and affordability compared to traditional porridges based on maize and finger millet.Maternal & child nutrition · 2025Article
- Three-dimensional spectrochromatographic determination of chlorogenic acid in Melampyrum stenophyllum Boiss. extracts by parallel factor analysis.Phytochemical analysis : PCA · 2025Article
- Comparative Analysis of the Biochemical and Molecular Responses ofMarine drugs · 2024Article
- Conserved carotenoid pigmentation in reproductive organs of Charophyceae.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2024Article
- An Integrated Approach for Biofortification of Carotenoids in Cowpea for Human Nutrition and Health.Plants (Basel, Switzerland) · 2024Review
- The Combining Ability and Heterosis Analysis of Sweet-Waxy Corn Hybrids for Yield-Related Traits and Carotenoids.Plants (Basel, Switzerland) · 2024Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe dietary carotenoids serve as precursor for vitamin A and prevent several chronic-degenerative diseases. The carotenoid profiling is necessary to understand their importance on human health. However, the available high-performance liquid chromatography (HPLC) methods to resolve the major carotenoids require longer analysis times and do not adequately resolve the violaxanthin and neoxanthin.
resultsA fast and sensitive HPLC method was developed using a C30 column at 20°C with a gradient consisting of methanol, methyl-tert-butyl ether and water. A total of 15 major carotenoids, including 14 all-trans forms and one cis form were resolved within 20 min. The method also distinctly resolved violaxanthin and neoxanthin present in green tissues. Additionally this method also resolved geometrical isomers of the carotenoids.
conclusionThe HPLC coupled with C30 column efficiently resolved fifteen carotenoids and their isomers in shorter runtime of 20 min. Application of this method to diverse matrices such as tomato fruits and leaves, Arabidopsis leaves and green pepper fruits showed the versatility and robustness of the method. The method would be useful for high throughput analysis of large number of samples.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.