Evidence map›Paper›PMID 25688272›Full record

ArticleStem cells international2015

Human adult stem cells maintain a constant phenotype profile irrespective of their origin, Basal media, and long term cultures.

Indumathi Somasundaram, Rashmi Mishra, Harikrishnan Radhakrishnan, Rajkumar Sankaran, Venkata Naga Srikanth Garikipati, Dhanasekaran Marappagounder

Open access · goldAbstract read
In one paragraph

Article in Stem cells international, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
1.2field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 11 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 5 institutions in 3 countries.

Indumathi SomasundaramDepartment of Stem Cells, National Institute of Nutrition, Secunderabad 500 007, India.
Rashmi MishraInstitute for Biochemistry and Molecular Biology, Ulm University, M24, Level 3, Room 358, Albert-Einstein-Allee 11, 89081 Ulm, Germany.
Harikrishnan RadhakrishnanBerlin School of Integrative Oncology, Buch, 131254 Berlin, Germany.
Rajkumar SankaranLifeline RIGID Hospitals, Chennai 600 010, India.
Venkata Naga Srikanth GarikipatiStem Cell Therapy Program, Center for Translational Medicine, Temple University School of Medicine, Temple University, Room MERB 9-943, 3500 North Broad Street, Philadelphia, PA 19104, USA.
Dhanasekaran MarappagounderRee Laboratories Private Limited, Andheri West, Mumbai 400 053, India.ORCID 0000-0003-2231-3238
Lifeline Hospital · INMSB Medical School Berlin · DENational Institute of Nutrition · INTemple University · USUniversität Ulm · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The study aims to identify the phenotypic marker expressions of different human adult stem cells derived from, namely, bone marrow, subcutaneous fat, and omentum fat, cultured in different media, namely, DMEM-Low Glucose, Alpha-MEM, DMEM-F12 and DMEM-KO and under long term culture conditions (>P20). We characterized immunophenotype by using various hematopoietic, mesenchymal, endothelial markers, and cell adhesion molecules in the long term cultures (Passages-P1, P3, P5, P9, P12, P15, and P20.) Interestingly, data revealed similar marker expression profiles irrespective of source, basal media, and extensive culturing. This demonstrates that all adult stem cell sources mentioned in this study share similar phenotypic marker and all media seem appropriate for culturing these sources. However, a disparity was observed in the markers such as CD49d, CD54, CD117, CD29, and CD106, thereby warranting further research on these markers. Besides the aforesaid objective, it is understood from the study that immunophenotyping acts as a valuable tool to identify inherent property of each cell, thereby leading to a valuable cell based therapy.

Identifiers

PMID25688272
PMCPMC4320880
OpenAlexW1990765837

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.