ArticleThe Journal of cell biology2015
Two Polo-like kinase 4 binding domains in Asterless perform distinct roles in regulating kinase stability.
Article in The Journal of cell biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 37 citations in OpenAlex.
- Unraveling the molecular details of centriole elongation.Trends open · 2026Article
- PLK4: Master Regulator of Centriole Duplication and Its Therapeutic Potential.Cytoskeleton (Hoboken, N.J.) · 2025Review
- Generating CRISPR-edited clonal lines of culturedBiology methods & protocols · 2024Article
- Polo-like kinase 4 homodimerization and condensate formation regulate its own protein levels but are not required for centriole assembly.Molecular biology of the cell · 2023Article
- Plk4 triggers autonomous de novo centriole biogenesis and maturation.The Journal of cell biology · 2021Article
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- Article
- CEP120 interacts with C2CD3 and Talpid3 and is required for centriole appendage assembly and ciliogenesis.Scientific reports · 2019Article
- Asterless is a Polo-like kinase 4 substrate that both activates and inhibits kinase activity depending on its phosphorylation state.Molecular biology of the cell · 2018Article
- Review
- A homeostatic clock sets daughter centriole size in flies.The Journal of cell biology · 2018Article
- An ordered pattern of Ana2 phosphorylation by Plk4 is required for centriole assembly.The Journal of cell biology · 2018Article
- Human microcephaly protein RTTN interacts with STIL and is required to build full-length centrioles.Nature communications · 2017Article
- NEK7 is required for G1 progression and procentriole formation.Molecular biology of the cell · 2017Article
- The Centrioles, Centrosomes, Basal Bodies, and Cilia ofGenetics · 2017Review
- Klp10A modulates the localization of centriole-associated proteins during Drosophila male gametogenesis.Cell cycle (Georgetown, Tex.) · 2016Article
- The PLK4-STIL-SAS-6 module at the core of centriole duplication.Biochemical Society transactions · 2016Review
- Quantitative analysis of human centrosome architecture by targeted proteomics and fluorescence imaging.The EMBO journal · 2016Article
- A centrosome interactome provides insight into organelle assembly and reveals a non-duplication role for Plk4.Nature communications · 2016Article
- Asterless is required for centriole length control and sperm development.The Journal of cell biology · 2016Article
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Authors and funding
9 authors at 5 institutions in 1 country.
Funding
Abstract
Plk4 (Polo-like kinase 4) and its binding partner Asterless (Asl) are essential, conserved centriole assembly factors that induce centriole amplification when overexpressed. Previous studies found that Asl acts as a scaffolding protein; its N terminus binds Plk4's tandem Polo box cassette (PB1-PB2) and targets Plk4 to centrioles to initiate centriole duplication. However, how Asl overexpression drives centriole amplification is unknown. In this paper, we investigated the Asl-Plk4 interaction in Drosophila melanogaster cells. Surprisingly, the N-terminal region of Asl is not required for centriole duplication, but a previously unidentified Plk4-binding domain in the C terminus is required. Mechanistic analyses of the different Asl regions revealed that they act uniquely during the cell cycle: the Asl N terminus promotes Plk4 homodimerization and autophosphorylation during interphase, whereas the Asl C terminus stabilizes Plk4 during mitosis. Therefore, Asl affects Plk4 in multiple ways to regulate centriole duplication. Asl not only targets Plk4 to centrioles but also modulates Plk4 stability and activity, explaining the ability of overexpressed Asl to drive centriole amplification.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.