ArticleJournal of proteome research2015
Monitoring newly synthesized proteins over the adult life span of Caenorhabditis elegans.
Article in Journal of proteome research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
12 citing papers in PubMed, 23 citations in OpenAlex.
- Integrative Quantitative Proteomics and N-Glycoproteomics Reveal Age-Processing of N-Glycosylation in Caenorhabditis elegans.Proteomics · 2026Article
- SETD2 regulates the methylation of translation elongation factor eEF1A1 in clear cell renal cell carcinoma.Kidney cancer journal : official journal of the Kidney Cancer Association · 2022Article
- Accumulation of "Old Proteins" and the Critical Need for MS-based Protein Turnover Measurements in Aging and Longevity.Proteomics · 2020Review
- Phosphorylation of the histone demethylase KDM5B and regulation of the phenotype of triple negative breast cancer.Scientific reports · 2019Article
- Multidimensional Proteomics Identifies Declines in Protein Homeostasis and Mitochondria as Early Signals for Normal Aging and Age-associated Disease inMolecular & cellular proteomics : MCP · 2019Article
- α-Defensin HD5 StabilizesPathogens & immunity · 2019Article
- Stable isotope-based flux studies in nonalcoholic fatty liver disease.Pharmacology & therapeutics · 2018Review
- Changes of Protein Turnover in AgingMolecular & cellular proteomics : MCP · 2017Article
- Nucleolar expansion and elevated protein translation in premature aging.Nature communications · 2017Article
- Monitoring the synthesis of biomolecules using mass spectrometry.Philosophical transactions. Series A, Mathematical, physical, and engineering sciences · 2016Article
- FOXO/DAF-16 Activation Slows Down Turnover of the Majority of Proteins in C. elegans.Cell reports · 2016Article
- Reciprocal Changes in Phosphoenolpyruvate Carboxykinase and Pyruvate Kinase with Age Are a Determinant of Aging in Caenorhabditis elegans.The Journal of biological chemistry · 2016Article
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
Abstract
Little is known regarding how the synthesis and degradation of individual proteins change during the life of an organism. Such knowledge is vital to understanding the aging process. To fill this knowledge gap, we monitored newly synthesized proteins on a proteome scale in Caenorhabditis elegans over time during adulthood using a stable-isotope labeling by amino acids in cell culture (SILAC)-based label-chase approach. For most proteins, the rate of appearance of newly synthesized protein was high during the first 5 days of adulthood, slowed down between the fifth and the 11th days, and then increased again after the 11th day. However, the magnitude of appearance rate differed significantly from protein to protein. For example, the appearance of newly synthesized protein was fast for proteins involved in embryonic development, transcription regulation, and lipid binding/transport, with >70% of these proteins newly synthesized by day 5 of adulthood, whereas it was slow for proteins involved in cellular assembly and motility, such as actin and myosin, with <70% of these proteins newly synthesized even on day 16. The late-life increase of newly synthesized protein was especially high for ribosomal proteins and ATP synthases. We also investigated the effect of RNAi-mediated knockdown of the rpl-9 (ribosomal protein), atp-3 (ATP synthase), and ril-1 (RNAi-induced longevity-1) genes and found that inhibiting the expression of atp-3 and ril-1 beginning in late adulthood is still effective to extend the life span of C. elegans.
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