ArticleJournal of the American Chemical Society2015
Biochemical and EPR-spectroscopic investigation into heterologously expressed vinyl chloride reductive dehalogenase (VcrA) from Dehalococcoides mccartyi strain VS.
Article in Journal of the American Chemical Society, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 81 citations in OpenAlex.
- Revisiting the dihaloelimination potential ofApplied and environmental microbiology · 2026Article
- Cofactor binding interactions with reductive dehalogenase shape cobamide utilization in organohalide-respiring bacteria.Communications biology · 2026Article
- Developing anApplied and environmental microbiology · 2026Article
- Novel chemistry and structural perspectives in vitamin BChemical science · 2026Review
- The PceA-orthologous reductive dehalogenase of Dehalococcoides mccartyi CBDB1 is involved in 1,2,4-trichlorobenzene respiration.FEMS microbiology ecology · 2026Article
- CO-driven electron and carbon flux fuels synergistic microbial reductive dechlorination.Microbiome · 2024Article
- Burning question: Rethinking organohalide degradation strategy for bioremediation applications.Microbial biotechnology · 2024Review
- Understanding the sources, function, and irreplaceable role of cobamides in organohalide-respiring bacteria.Frontiers in microbiology · 2024Review
- Article
- Heterologous Expression of ActiveApplied and environmental microbiology · 2022Article
- Interspecies metabolite transfer and aggregate formation in a co-culture of Dehalococcoides and Sulfurospirillum dehalogenating tetrachloroethene to ethene.The ISME journal · 2021Article
- Biological effects of tourmaline treatment onRSC advances · 2021Article
- Integrating single-cobalt-site and electric field of boron nitride in dechlorination electrocatalysts by bioinspired design.Nature communications · 2021Article
- Dual Element (C/Cl) Isotope Analysis Indicates Distinct Mechanisms of Reductive Dehalogenation of Chlorinated Ethenes and Dichloroethane inFrontiers in microbiology · 2020Article
- Reductive dechlorination of 1,2-dichloroethane in the presence of chloroethenes and 1,2-dichloropropane as co-contaminants.Applied microbiology and biotechnology · 2019Article
- Guided cobamide biosynthesis for heterologous production of reductive dehalogenases.Microbial biotechnology · 2019Article
- An Aminoimidazole Radical Intermediate in the Anaerobic Biosynthesis of the 5,6-Dimethylbenzimidazole Ligand to Vitamin B12.Journal of the American Chemical Society · 2018Article
- Stereochemical and Mechanistic Investigation of the Reaction Catalyzed by Fom3 from Streptomyces fradiae, a Cobalamin-Dependent Radical S-Adenosylmethionine Methylase.Biochemistry · 2018Article
- Selective Utilization of Benzimidazolyl-Norcobamides as Cofactors by the Tetrachloroethene Reductive Dehalogenase of Sulfurospirillum multivorans.Journal of bacteriology · 2018Article
- Functional Expression and Characterization of Tetrachloroethene Dehalogenase FromFrontiers in microbiology · 2018Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Reductive dehalogenases play a critical role in the microbial detoxification of aquifers contaminated with chloroethenes and chlorethanes by catalyzing the reductive elimination of a halogen. We report here the first heterologous production of vinyl chloride reductase VcrA from Dehalococcoides mccartyi strain VS. Heterologously expressed VcrA was reconstituted to its active form by addition of hydroxocobalamin/adenosylcobalamin, Fe(3+), and sulfide in the presence of mercaptoethanol. The kinetic properties of reconstituted VcrA catalyzing vinyl chloride reduction with Ti(III)-citrate as reductant and methyl viologen as mediator were similar to those obtained previously for VcrA as isolated from D. mccartyi strain VS. VcrA was also found to catalyze a novel reaction, the environmentally important dihaloelimination of 1,2-dichloroethane to ethene. Electron paramagnetic resonance (EPR) spectroscopic studies with reconstituted VcrA in the presence of mercaptoethanol revealed the presence of Cob(II)alamin. Addition of Ti(III)-citrate resulted in the appearance of a new signal characteristic of a reduced [4Fe-4S] cluster and the disappearance of the Cob(II)alamin signal. UV-vis absorption spectroscopy of Ti(III)citrate-treated samples revealed the formation of two new absorption maxima characteristic of Cob(I)alamin. No evidence for the presence of a [3Fe-4S] cluster was found. We postulate that during the reaction cycle of VcrA, a reduced [4Fe-4S] cluster reduces Co(II) to Co(I) of the enzyme-bound cobalamin. Vinyl chloride reduction to ethene would be initiated when Cob(I)alamin transfers an electron to the substrate, generating a vinyl radical as a potential reaction intermediate.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.