ArticleNature structural & molecular biology2015
Crystal structure of a phosphorylation-coupled vitamin C transporter.
Article in Nature structural & molecular biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- A trimeric architecture reveals the glucitol PTS transporter as a distinct superfamily.Communications biology · 2026Article
- Cell-Penetrating Peptide-Mediated Biomolecule Transportation in Artificial Lipid Vesicles and Living Cells.Molecules (Basel, Switzerland) · 2024Review
- Roles of adenine methylation in the physiology of Lacticaseibacillus paracasei.Nature communications · 2023Article
- Structural basis of vitamin C recognition and transport by mammalian SVCT1 transporter.Nature communications · 2023Article
- Novel Multifunctional Ascorbic Triazole Derivatives for Amyloidogenic Pathway Inhibition, Anti-Inflammation, and Neuroprotection.Molecules (Basel, Switzerland) · 2021Article
- Transporters of glucose and other carbohydrates in bacteria.Pflugers Archiv : European journal of physiology · 2020Review
- Elevator-type mechanisms of membrane transport.Biochemical Society transactions · 2020Review
- Multiscale Simulations of Biological Membranes: The Challenge To Understand Biological Phenomena in a Living Substance.Chemical reviews · 2019Article
- Article
- Structure of an EIIC sugar transporter trapped in an inward-facing conformation.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Agrocin 108 is a 5'-cytidine nucleotide bacteriocin containing a carbocyclic phosphoryl-ascorbate group.The Journal of antibiotics · 2018Article
- Human bile acid transporter ASBT (SLC10A2) forms functional non-covalent homodimers and higher order oligomers.Biochimica et biophysica acta. Biomembranes · 2018Article
- Insights into the molecular mechanism of dehalogenation catalyzed by D-2-haloacid dehalogenase from crystal structures.Scientific reports · 2018Article
- Inward-facing conformation of l-ascorbate transporter suggests an elevator mechanism.Cell discovery · 2018Article
- The V-motifs facilitate the substrate capturing step of the PTS elevator mechanism.Journal of structural biology · 2016Article
- The Structure of a Sugar Transporter of the Glucose EIIC Superfamily Provides Insight into the Elevator Mechanism of Membrane Transport.Structure (London, England : 1993) · 2016Article
- Time to Stop Holding the Elevator: A New Piece of the Transport Protein Mechanism Puzzle.Structure (London, England : 1993) · 2016Article
- Transport protein evolution deduced from analysis of sequence, topology and structure.Current opinion in structural biology · 2016Review
- Structure of eukaryotic purine/H(+) symporter UapA suggests a role for homodimerization in transport activity.Nature communications · 2016Article
- Crystal structures reveal the molecular basis of ion translocation in sodium/proton antiporters.Nature structural & molecular biology · 2016Article
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacteria use vitamin C (L-ascorbic acid) as a carbon source under anaerobic conditions. The phosphoenolpyruvate-dependent phosphotransferase system (PTS), comprising a transporter (UlaA), a IIB-like enzyme (UlaB) and a IIA-like enzyme (UlaC), is required for the anaerobic uptake of vitamin C and its phosphorylation to L-ascorbate 6-phosphate. Here, we present the crystal structures of vitamin C-bound UlaA from Escherichia coli in two conformations at 1.65-Å and 2.35-Å resolution. UlaA forms a homodimer and exhibits a new fold. Each UlaA protomer consists of 11 transmembrane segments arranged into a 'V-motif' domain and a 'core' domain. The V motifs form the interface between the two protomers, and the core-domain residues coordinate vitamin C. The alternating access of the substrate from the opposite side of the cell membrane may be achieved through rigid-body rotation of the core relative to the V motif.
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