ArticleMolecules (Basel, Switzerland)2015
Structural analysis of metabolites of asiatic acid and its analogue madecassic acid in zebrafish using LC/IT-MSn.
Article in Molecules (Basel, Switzerland), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Network pharmacology and in vivo experimental analysis for validating the antifibrotic potential of Punica granatum leaves against skin scleroderma: the role of oxidative stress, inflammation and TGF-β1/Snail 1/p-Smad 3 signaling pathway.Inflammopharmacology · 2026Article
- Comparative Metabolomics and Network Pharmacology Analysis Reveal Shared Neuroprotective Mechanisms of Bacopa monnieri (L.) Wettst and Centella asiatica (L.) Urb.Molecular neurobiology · 2024Article
- How to Promote Skin Repair? In-Depth Look at Pharmaceutical and Cosmetic Strategies.Pharmaceuticals (Basel, Switzerland) · 2023Article
- Rapid Separation of Asiatic Acid, Quercetin, and Kaempferol from Traditional Chinese MedicineInternational journal of analytical chemistry · 2023Article
- UPLC-QTOF-MS Based Comparison of Rotundic Acid Metabolic Profiles in Normal and NAFLD Rats.Metabolites · 2022Article
- Structural Investigation of Betulinic Acid Plasma Metabolites by Tandem Mass Spectrometry.Molecules (Basel, Switzerland) · 2022Article
- Alchemilla viridiflora Rothm.: the potent natural inhibitor of angiotensin I-converting enzyme.Molecular and cellular biochemistry · 2022Article
- A Validated HPLC-PDA-HRMS Method to Investigate the Biological Stability and Metabolism of Antiparasitic Triterpenic Esters.Molecules (Basel, Switzerland) · 2021Article
- Insight into the Secondary Metabolites ofPlants (Basel, Switzerland) · 2021Article
- Pentacyclic triterpenoid-rich fraction of the Hardy kiwi (Actinidia arguta) improves brain dysfunction in high fat diet-induced obese mice.Scientific reports · 2020Article
- Article
- Pharmacological Properties, Molecular Mechanisms, and Pharmaceutical Development of Asiatic Acid: A Pentacyclic Triterpenoid of Therapeutic Promise.Frontiers in pharmacology · 2018Review
- LC-MS/MS Tandem Mass Spectrometry for Analysis of Phenolic Compounds and Pentacyclic Triterpenes in Antifungal Extracts of Terminalia brownii (Fresen).Antibiotics (Basel, Switzerland) · 2017Article
- Pharmacological effects of asiatic acid in glioblastoma cells under hypoxia.Molecular and cellular biochemistry · 2017Article
- Rapid Screening for α-Glucosidase Inhibitors fromFrontiers in pharmacology · 2017Article
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Authors and funding
6 authors.
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Abstract
Although zebrafish has become a significant animal model for drug discovery and screening, drug metabolism in zebrafish remains largely unknown. Asiatic acid (AA) and madecassic acid (MA), two natural pentacyclic triterpenoids mainly obtained from Centella asiatica (L.) Urban, have been found to possess many pharmacological effects. This study is to probe the metabolic capability of zebrafish via investigation of the drug metabolism of AA and MA in zebrafish, using a sensitive LC/IT-MSn method. In addition, the main fragmentation pathways of AA and MA were reported for the first time. Nineteen metabolites of AA and MA were firstly identified after zebrafish was exposed to the drug, which all were the phase I metabolites and mainly formed from hydroxylation, dehydrogenation, hydroxylation and dehydrogenation, dihydroxylation and dehydrogenation, and dehydroxylation reaction. The results indicated that zebrafish possessed strong metabolic capacity, and the metabolites of AA and MA were formed via similar metabolic pathways and well matched with the known metabolic rules in vivo and in vitro, which supports the widely use of this system in drug metabolism research. This investigation would also contribute to the novel information on the structural elucidation, in vivo metabolites and metabolic mechanism of pentacyclic triterpenoids.
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