ArticleFEBS open bio2015
Creation of targeted genomic deletions using TALEN or CRISPR/Cas nuclease pairs in one-cell mouse embryos.
Article in FEBS open bio, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 37 citations in OpenAlex.
- A deletion containing a CTCF-element in intron 8 of the Bbs7 gene is partially responsible for juvenile obesity in the Berlin Fat Mouse.Mammalian genome : official journal of the International Mammalian Genome Society · 2022Article
- Recent Advances in the Production of Genome-Edited Rats.International journal of molecular sciences · 2022Review
- Deficiency of the innate immune adaptor STING promotes autoreactive T cell expansion in NOD mice.Diabetologia · 2021Article
- Toward precise CRISPR DNA fragment editing and predictable 3D genome engineering.Journal of molecular cell biology · 2021Review
- Mutant non-coding RNA resource in mouse embryonic stem cells.Disease models & mechanisms · 2021Article
- Efficient mutagenesis targeting theAmerican journal of translational research · 2021Article
- A homology independent sequence replacement strategy in human cells using a CRISPR nuclease.Open biology · 2021Article
- GPRIN3 Controls Neuronal Excitability, Morphology, and Striatal-Dependent Behaviors in the Indirect Pathway of the Striatum.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2019Article
- Rapid Generation of Long Noncoding RNA Knockout Mice Using CRISPR/Cas9 Technology.Non-coding RNA · 2019Article
- The Trem2 R47H Alzheimer's risk variant impairs splicing and reduces Trem2 mRNA and protein in mice but not in humans.Molecular neurodegeneration · 2018Article
- The Application of CRISPR/Cas9 for the Treatment of Retinal Diseases.The Yale journal of biology and medicine · 2017Review
- GATA2/3-TFAP2A/C transcription factor network couples human pluripotent stem cell differentiation to trophectoderm with repression of pluripotency.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- The FTD-like syndrome causing TREM2 T66M mutation impairs microglia function, brain perfusion, and glucose metabolism.The EMBO journal · 2017Article
- Functional interrogation of non-coding DNA through CRISPR genome editing.Methods (San Diego, Calif.) · 2017Review
- Novel impact of the DNMT3A R882H mutation on GSH metabolism in a K562 cell model established by TALENs.Oncotarget · 2017Article
- CRISPR/Cas9-Mediated Genome Editing as a Therapeutic Approach for Leber Congenital Amaurosis 10.Molecular therapy : the journal of the American Society of Gene Therapy · 2017Article
- TALEN-mediated genome-editing approaches in the liverwortPlant methods · 2017Article
- To CRISPR and beyond: the evolution of genome editing in stem cells.Regenerative medicine · 2016Review
- Enhanced genome editing in mammalian cells with a modified dual-fluorescent surrogate system.Cellular and molecular life sciences : CMLS · 2016Article
- An alternative strategy for targeted gene replacement in plants using a dual-sgRNA/Cas9 design.Scientific reports · 2016Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The use of TALEN and CRISPR/CAS nucleases is becoming increasingly popular as a means to edit single target sites in one-cell mouse embryos. Nevertheless, an area that has received less attention concerns the engineering of structural genome variants and the necessary religation of two distant double-strand breaks. Herein, we applied pairs of TALEN or sgRNAs and Cas9 to create deletions in the Rab38 gene. We found that the deletion of 3.2 or 9.3 kb, but not of 30 kb, occurs at a frequency of 6-37%. This is sufficient for the direct production of mutants by embryo microinjection. Therefore, deletions up to ∼10 kb can be readily achieved for modeling human disease alleles. This work represents an important step towards the establishment of new protocols that support the ligation of remote DSB ends to achieve even larger rearrangements.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.