Evidence map›Paper›PMID 25685469›Full record

ArticleJournal of advanced research2014

Methylation of multiple genes in hepatitis C virus associated hepatocellular carcinoma.

Abdel-Rahman N Zekri, Abeer A Bahnasy, Fatma Elzahraa M Shoeab, Waleed S Mohamed, Dina H El-Dahshan, Fahmey T Ali, Gilane M Sabry, Nairajana Dasgupta, Sayed S Daoud

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of advanced research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
27citing papers in PubMed, 2 pooled it
1.4field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

27 citing papers in PubMed, 2 syntheses or guidelines pooled it, 44 citations in OpenAlex.

  1. Pooled it
  2. Pooled it
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  4. Review
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  8. Review
  9. Article
  10. MGMT-Methylation in Non-Neoplastic Diseases of the Central Nervous System.International journal of molecular sciences · 2021
    Article
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  14. Article
  15. Article
  16. Article
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  18. Clinicopathological and prognostic significance ofCancer management and research · 2019
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 5 institutions in 2 countries.

Abdel-Rahman N ZekriVirology and Immunology Unit, Cancer Biology Department, National Cancer Institute, Cairo University, Egypt.
Abeer A BahnasyPathology Department, National Cancer Institute, Cairo University, Egypt.
Fatma Elzahraa M ShoeabFaculty of Science, Ain Shams, University, Egypt.
Waleed S MohamedVirology and Immunology Unit, Cancer Biology Department, National Cancer Institute, Cairo University, Egypt.
Dina H El-DahshanFaculty of Medicine, Beni Suef University, Egypt.
Fahmey T AliFaculty of Science, Ain Shams, University, Egypt.
Gilane M SabryFaculty of Science, Ain Shams, University, Egypt.
Nairajana DasguptaCenter for Integrated Biotechnology, Washington State University, Pullman, WA, United States.
Sayed S DaoudCenter for Integrated Biotechnology, Washington State University, Pullman, WA, United States.
Ain Shams University · EGCairo University · EGWashington State University · USBeni-Suef University · EGChildren Cancer Hospital · EG

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We studied promoter methylation (PM) of 11 genes in Peripheral Blood Lymphocytes (PBLs) and tissues of hepatitis C virus (HCV) associated hepatocellular carcinoma (HCC) and chronic hepatitis (CH) Egyptian patients. The present study included 31 HCC with their ANT, 38 CH and 13 normal hepatic tissue (NHT) samples. In all groups, PM of APC, FHIT, p15, p73, p14, p16, DAPK1, CDH1, RARβ, RASSF1A, O(6)MGMT was assessed by methylation-specific PCR (MSP). APC and O6-MGMT protein expression was assessed by immunohistochemistry (IHC) in the studied HCC and CH (20 samples each) as well as in a different HCC and CH set for confirmation of MSP results. PM was associated with progression from CH to HCC. Most genes showed high methylation frequency (MF) and the methylation index (MI) increased with disease progression. MF of p14, p73, RASSF1A, CDH1 and O(6)MGMT was significantly higher in HCC and their ANT. MF of APC was higher in CH. We reported high concordance between MF in HCC and their ANT, MF in PBL and CH tissues as well as between PM and protein expression of APC and O(6)MGMT. A panel of 4 genes (APC, p73, p14, O(6)MGMT) classifies the cases independently into HCC and CH with high accuracy (89.9%), sensitivity (83.9%) and specificity (94.7%). HCV infection may contribute to hepatocarcinogenesis through enhancing PM of multiple genes. PM of APC occurs early in the cascade while PM of p14, p73, RASSF1A, RARB, CDH1 and O(6)MGMT are late changes. A panel of APC, p73, p14, O6-MGMT could be used in monitoring CH patients for early detection of HCC. Also, we found that, the methylation status is not significantly affected by whether the tissue was from the liver or PBL, indicating the possibility of use PBL as indicator to genetic profile instead of liver tissue regardless the stage of disease.

Indexed as

Chronic hepatitisHepatitis C virus-genotype 4Hepatocellular carcinomaPromoter methylation

Identifiers

PMID25685469
PMCPMC4294722
OpenAlexW1963822977

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.