ArticleMolecular vision2015
Chondrogenesis in scleral stem/progenitor cells and its association with form-deprived myopia in mice.
Article in Molecular vision, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers, 1 of them a synthesis that pooled it.
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Who cites it
17 citing papers in PubMed, 1 synthesis or guideline pooled it, 29 citations in OpenAlex.
- A Review of Current Concepts of the Etiology and Treatment of Myopia.Eye & contact lens · 2018Pooled it
- Timp2-modified gelatinhydroxyphenylpropionic acid hydrogels reverse enhanced scleral recovery and suppress myopia development in mice.Materials today. Bio · 2025Article
- Low-level red light inhibits human retinal pigment epithelial cell fibrosis via UBE2C in a myopia-simulating hypoxic microenvironment.European journal of medical research · 2025Article
- Distinct Transcriptomic Profiles of Cultured Anterior and Posterior Populations of Human Infant Scleral Fibroblasts: Including Dopamine Receptors.Investigative ophthalmology & visual science · 2025Article
- What is the relationship between the choroidal vascularity ındex (CVI) and myopia?International ophthalmology · 2025Article
- Association of choroidal thickness and blood flow features with asymmetric axial lengths in children with unilateral myopic anisometropia.BMC ophthalmology · 2024Article
- The human lens is capable of trilineage differentiation towards osteo-, chondro-, and adipogenesis-a model for studying cataract pathogenesis.Frontiers in bioengineering and biotechnology · 2023Article
- RNA-sequencing analysis reveals the long noncoding RNA profile in the mouse myopic retina.Frontiers in genetics · 2022Article
- Choroidal thickness and choriocapillaris vascular density in myopic anisometropia.Eye and vision (London, England) · 2021Article
- SLC39A5 dysfunction impairs extracellular matrix synthesis in high myopia pathogenesis.Journal of cellular and molecular medicine · 2021Article
- Comparative proteome analysis of form-deprivation myopia in sclera with iTRAQ-based quantitative proteomics.Molecular vision · 2021Article
- A role of color vision in emmetropization in C57BL/6J mice.Scientific reports · 2020Article
- Dynamic changes of periostin and collagen I in the sclera during progressive myopia in guinea pigs.Arquivos brasileiros de oftalmologia · 2020Article
- All-trans retinoic acid stimulates the secretion of TGF-β2 via the phospholipase C but not the adenylyl cyclase signaling pathway in retinal pigment epithelium cells.BMC ophthalmology · 2019Article
- Systematic Analysis of Transcriptomic Profile of the Effects of Low Dose Atropine Treatment on Scleral Fibroblasts using Next-Generation Sequencing and Bioinformatics.International journal of medical sciences · 2019Article
- Scleral hypoxia is a target for myopia control.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Silencing of Wnt5a prevents interleukin-1β-induced collagen type II degradation in rat chondrocytes.Experimental and therapeutic medicine · 2016Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
Abstract
purposePreviously, we demonstrated that scleral stem/progenitor cells (SSPCs) from mice have a chondrogenic differentiation potential, which is stimulated by transforming growth factor-β (TGF-β). In the present study, we hypothesized that chondrogenesis in the sclera could be a possible mechanism in myopia development. Therefore, we investigated the association of form-deprivation myopia (FDM) with expressions in mice sclera representing the chondrogenic phenotype: collagen type II (Col2) and α-smooth muscle actin (α-SMA).
methodsThe mRNA levels of α-SMA and Col2 in cultured murine SSPCs during chondrogenesis stimulated by TGF-β2 were determined by real-time quantitative RT-PCR (qRT-PCR). The expression patterns of α-SMA and Col2 were assessed by immunohistochemistry in a three dimensional pellet culture. In an FDM mouse model, a western blot analysis and immunofluorescence study were used to detect the changes in the α-SMA and Col2 protein expressions in the sclera. In the RPE-choroid complex, qRT-PCR was used to detect any changes in the TGF-β mRNA expression.
resultsThe treatment of SSPCs in vitro with TGF-β2 for 24 h at 1 or 10 ng/ml led to increased levels of both the α-SMA and Col2 expressions. In addition, we observed the formation of cartilage-like pellets from TGF-β2-treated SSPCs. Both α-SMA and Col2 were expressed in the pellet. In an in-vivo study, the α-SMA and Col2 protein expressions were significantly increased in the sclera of FDM eyes in comparison to contralateral control eyes. Similarly, the levels of TGF-β in the RPE-choroid complex of an FDM eye were also significantly elevated.
conclusionBased on the concept of stem cells possessing multipotent differentiation potentials, scleral chondrogenesis induced by SSPCs may play a role in myopia development. The increased expressions of the cartilage-associated proteins Col2 and α-SMA during scleral chondrogenesis may be potential markers for myopia development. In addition, the increased levels of TGF-β mRNA in the RPE-choroid complex might induce the chondrogenic change in the sclera during myopia development.
Indexed as
Identifiers
25684979PMC4323720W2215841289What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.