Evidence map›Paper›PMID 25684227›Full record

ArticleStem cell reports2015

The BET family member BRD4 interacts with OCT4 and regulates pluripotency gene expression.

Tao Wu, Hugo Borges Pinto, Yasunao F Kamikawa, Mary E Donohoe

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.

0numbers the graph read from it
0cells of the map it votes in
50citing papers in PubMed
5.4field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

50 citing papers in PubMed, 84 citations in OpenAlex.

  1. Review
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  12. Super-enhancers conserved within placental mammals maintain stem cell pluripotency.Proceedings of the National Academy of Sciences of the United States of America · 2022
    Article
  13. Article
  14. Article
  15. Article
  16. An interplay between BRD4 and G9a regulates skeletal myogenesis.Frontiers in cell and developmental biology · 2022
    Article
  17. Article
  18. What do Transcription Factors Interact With?Journal of molecular biology · 2021
    Review
  19. Mammalian DNA Replication Timing.Cold Spring Harbor perspectives in biology · 2021
    Review
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Tao WuBurke Medical Research Institute, 785 Mamaroneck Avenue, White Plains, NY 10605, USA; Departments of Neuroscience and Cell and Developmental Biology, Weill Cornell Medical College, New York, NY 10065, USA.
Hugo Borges PintoBurke Medical Research Institute, 785 Mamaroneck Avenue, White Plains, NY 10605, USA; Departments of Neuroscience and Cell and Developmental Biology, Weill Cornell Medical College, New York, NY 10065, USA.
Yasunao F KamikawaBurke Medical Research Institute, 785 Mamaroneck Avenue, White Plains, NY 10605, USA; Departments of Neuroscience and Cell and Developmental Biology, Weill Cornell Medical College, New York, NY 10065, USA.
Mary E DonohoeBurke Medical Research Institute, 785 Mamaroneck Avenue, White Plains, NY 10605, USA; Departments of Neuroscience and Cell and Developmental Biology, Weill Cornell Medical College, New York, NY 10065, USA. Electronic address: med2008@med.cornell.edu.
Cornell University · USBurke Medical Research Institute · US

Funding

Allelic Choice in Rett SyndromeR01MH090267 · NIMH · WINIFRED MASTERSON BURKE MED RES INST · PI DONOHOE, MARY E · 2010 to 2014
$1.9M
NIMH NIH HHS R01 MH 090267NIMH NIH HHS R01 MH090267
6 · The paper itself

Abstract

Embryonic stem cell (ESC) pluripotency is controlled by defined transcription factors. During cellular differentiation, ESCs undergo a global epigenetic reprogramming. Female ESCs exemplify this process as one of the two X-chromosomes is globally silenced during X chromosome inactivation (XCI) to balance the X-linked gene disparity with XY males. The pluripotent factor OCT4 regulates XCI by triggering X chromosome pairing and counting. OCT4 directly binds Xite and Tsix, which encode two long noncoding RNAs (lncRNAs) that suppress the silencer lncRNA, Xist. To control its activity as a master regulator in pluripotency and XCI, OCT4 must have chromatin protein partners. Here we show that BRD4, a member of the BET protein subfamily, interacts with OCT4. BRD4 occupies the regulatory regions of pluripotent genes and the lncRNAs of XCI. BET inhibition or depletion of BRD4 reduces the expression of many pluripotent genes and shifts cellular fate showing that BRD4 is pivotal for transcription in ESCs.

Indexed as

Gene Expression Regulation, DevelopmentalAnimalsBromodomain Containing ProteinsCell DifferentiationCell LineCell Self RenewalEmbryonic Stem CellsEpigenesis, GeneticFemaleGene ExpressionGene SilencingHeat-Shock ProteinsMaleMiceNuclear ProteinsOctamer Transcription Factor-3Brd4 protein, mouseBromodomain Containing ProteinsHeat-Shock ProteinsHexim1 protein, mouseNuclear ProteinsOctamer Transcription Factor-3Positive Transcriptional Elongation Factor BRNA-Binding ProteinsRNA, Long NoncodingSesn3 protein, mouseSestrinsTranscription FactorsXIST non-coding RNA

Identifiers

PMID25684227
PMCPMC4375790
OpenAlexW2000965208

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.