ArticleBiotechnology progress
Functional myotube formation from adult rat satellite cells in a defined serum-free system.
Article in Biotechnology progress. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
12 citing papers in PubMed.
- Towards Cost-Effective and Sustainable Media Formulations for Terrestrial and Aquatic Cellular Agriculture.Foods (Basel, Switzerland) · 2026Review
- Development of a functional sarcopenia model utilizing a microcantilever microphysiological system as a phenotypic disease model.In vitro models · 2026Article
- Serum-free cultured meat production by usingiScience · 2025Article
- Mimicking the Tendon Microenvironment to Enhance Skeletal Muscle Adhesion and Longevity in a Functional Microcantilever Platform.ACS biomaterials science & engineering · 2023Article
- A Beefy-R culture medium: Replacing albumin with rapeseed protein isolates.Biomaterials · 2023Article
- Tissue Engineering Challenges for Cultivated Meat to Meet the Real Demand of a Global Market.International journal of molecular sciences · 2023Review
- Simple and effective serum-free medium for sustained expansion of bovine satellite cells for cell cultured meat.Communications biology · 2022Article
- A Human-Based Functional NMJ System for Personalized ALS Modeling and Drug Testing.Advanced therapeutics · 2020Article
- Differential Monocyte Actuation in a Three-Organ Functional Innate Immune System-on-a-Chip.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2020Article
- Myogenic differentiation of primary myoblasts and mesenchymal stromal cells under serum-free conditions on PCL-collagen I-nanoscaffolds.BMC biotechnology · 2018Article
- Engineered Human Contractile Myofiber Sheets as a Platform for Studies of Skeletal Muscle Physiology.Scientific reports · 2018Article
- Design, evaluation, and application of engineered skeletal muscle.Methods (San Diego, Calif.) · 2016Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
This manuscript describes the development of a culture system whereby mature contracting myotubes were formed from adult rat derived satellite cells. Satellite cells, extracted from the Tibialis Anterior of adult rats, were grown in defined serum-free growth and differentiation media, on a nonbiological substrate, N-1[3-trimethoxysilyl propyl] diethylenetriamine. Myotubes were evaluated morphologically and immunocytochemically, using MyHC specific antibodies, as well as functionally using patch clamp electrophysiology to measure ion channel activity. Results indicated the establishment of the rapid expression of adult myosin isoforms that contrasts to their slow development in embryonic cultures. This culture system has applications in the understanding and treatment of age-related muscle myopathy, muscular dystrophy, and for skeletal muscle engineering by providing a more relevant phenotype for both in vitro and in vivo applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.