ArticleThe Journal of cell biology1989
Role of microtubules in polarized delivery of apical membrane proteins to the brush border of the intestinal epithelium.
Article in The Journal of cell biology, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
61 citing papers in PubMed, 241 citations in OpenAlex.
- Microtubule organization and tubulin post-translational modifications in intact tissues and during regeneration in calcareous sponges.Cell and tissue research · 2025Article
- Altered Posttranslational Modification of Microtubules Contributes to Disturbed Enterocyte Morphology in Celiac Disease.International journal of molecular sciences · 2023Article
- Genetically induced microtubule disruption in the mouse intestine impairs intracellular organization and transport.Molecular biology of the cell · 2018Article
- Intestinal epithelial cell polarity defects in disease: lessons from microvillus inclusion disease.Disease models & mechanisms · 2018Review
- Microtubules regulate brush border formation.Journal of cellular physiology · 2018Article
- Microtubule organization, dynamics and functions in differentiated cells.Development (Cambridge, England) · 2017Review
- Disruption of Rab8a and Rab11a causes formation of basolateral microvilli in neonatal enteropathy.Journal of cell science · 2017Article
- Contraction of endothelial cells: 40 years of research, but the debate still lives.Histochemistry and cell biology · 2016Review
- Towards understanding microvillus inclusion disease.Molecular and cellular pediatrics · 2016Article
- Microtubules are required for efficient epithelial tight junction homeostasis and restoration.American journal of physiology. Cell physiology · 2014Article
- Polarizing intestinal epithelial cells electrically through Ror2.Journal of cell science · 2014Article
- Myosin Vb uncoupling from RAB8A and RAB11A elicits microvillus inclusion disease.The Journal of clinical investigation · 2014Article
- SOX8 regulates permeability of the blood-testes barrier that affects adult male fertility in the mouse.Biology of reproduction · 2013Article
- Shear stress-induced changes of membrane transporter localization and expression in mouse proximal tubule cells.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Galectin-3, a novel centrosome-associated protein, required for epithelial morphogenesis.Molecular biology of the cell · 2010Article
- Vav proteins are necessary for correct differentiation of mouse cecal and colonic enterocytes.Journal of cell science · 2009Article
- Tight junctions and the modulation of barrier function in disease.Histochemistry and cell biology · 2008Review
- Mammalian PAR-1 determines epithelial lumen polarity by organizing the microtubule cytoskeleton.The Journal of cell biology · 2004Article
- PepT1-mediated epithelial transport of dipeptides and cephalexin is enhanced by luminal leptin in the small intestine.The Journal of clinical investigation · 2001Article
- Rotavirus infection induces cytoskeleton disorganization in human intestinal epithelial cells: implication of an increase in intracellular calcium concentration.Journal of virology · 2000Article
1 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Colchicine- and vinblastine-induced depolymerization of microtubules (MTs) in the intestinal epithelium of rats and mice resulted in significant delivery of three apical membrane proteins (alkaline phosphatase, sucrase-isomaltase, and aminopeptidase N) to the basolateral membrane domain. In addition, typical brush borders (BBs) occurred at the basolateral cell surface, consisting of numerous microvilli that contained the four major components of the cytoskeleton of apical microvilli (actin, villin, fimbrin, and the 110-kD protein). Formation of basolateral microvilli required polymerization of actin and proceeded at glycocalyx-studded plaques that resembled the dense plaques located at the tips of apical microvilli. BBs from the basolateral membrane became internalized into BB-containing vacuoles which served as recipient organelles for newly synthesized apical membrane proteins. The BB vacuoles fused with each other and finally were inserted into the apical BB. Polarized distribution of Na+,K+-ATPase, a basolateral membrane protein, was not affected by drug-induced depolymerization of MTs. These observations indicate that Golgi-derived carrier vesicles (CVs) containing apical membrane proteins are vectorially guided to the apical cell surface by a retrograde transport along MTs. MTs are uniformly oriented towards a narrow space underneath the apical terminal web (termed subterminal space) that contains MT-organizing properties and controls polarized alignment of MTs. In contrast to apical CVs, targeting of basolateral CVs appears to be independent of MTs but demands a barrier at the apical membrane domain that prevents basolateral CVs from apical fusion (transport barrier hypothesis).
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