Evidence map›Paper›PMID 25682374›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2016

Pulse Field Gel Electrophoresis.

Batu K Sharma-Kuinkel, Thomas H Rude, Vance G Fowler

Open access · greenAbstract read
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
24citing papers in PubMed, 1 pooled it
6.9field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

24 citing papers in PubMed, 1 synthesis or guideline pooled it, 69 citations in OpenAlex.

  1. Programmed cell death detection methods: a systematic review and a categorical comparison.Apoptosis : an international journal on programmed cell death · 2022
    Pooled it
  2. Exploring clonality ofMicrobiology spectrum · 2026
    Article
  3. Review
  4. Article
  5. Article
  6. Urinary tract infections withArchive of clinical cases · 2024
    Review
  7. Review
  8. Review
  9. Clinical and Molecular Analyses of Recurrent Gram-Negative Bloodstream Infections.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2023
    Article
  10. Article
  11. Article
  12. A Simple, Robust, and Cost-effective Method for Genotyping Small-scale Mutations.Journal of clinical and translational pathology · 2022
    Article
  13. Review
  14. Microbial genomics · 2021
    Article
  15. Risk Factors for Recurrent Staphylococcus aureus Bacteremia.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2021
    Article
  16. Article
  17. Article
  18. Molecular Epidemiology ofAntibiotics (Basel, Switzerland) · 2020
    Review
  19. Article
  20. Differential Genotyping ofMicroorganisms · 2020
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 1 country.

Batu K Sharma-KuinkelDivision of Infectious Diseases, Department of Medicine, Duke University Medical Center, Box 102359, Medical Center, Durham, NC, 27710, USA.
Thomas H RudeDivision of Infectious Diseases, Department of Medicine, Duke University Medical Center, Box 102359, Medical Center, Durham, NC, 27710, USA.
Vance G FowlerDivision of Infectious Diseases, Department of Medicine, Duke University Medical Center, Box 102359, Medical Center, Durham, NC, 27710, USA. fowle003@mc.duke.edu.
Duke University Hospital · USDuke Medical Center · US

Funding

Host Susceptibility to S. aureusR01AI068804 · NIAID · DUKE UNIVERSITY · PI FOWLER, VANCE G. · 2007 to 2016
$6.9M
Mentoring Patient Oriented Research in Antibiotic-Resistant BacteriaK24AI093969 · NIAID · DUKE UNIVERSITY · PI FOWLER, VANCE G. · 2011 to 2020
$1.6M
NIAID NIH HHS K24 AI093969NIAID NIH HHS R01 AI068804
6 · The paper itself

Abstract

Pulse Field Gel Electrophoresis (PFGE) is a powerful genotyping technique used for the separation of large DNA molecules (entire genomic DNA) after digesting it with unique restriction enzymes and applying to a gel matrix under the electric field that periodically changes direction. PFGE is a variation of agarose gel electrophoresis that permits analysis of bacterial DNA fragments over an order of magnitude larger than that with conventional restriction enzyme analysis. It provides a good representation of the entire bacterial chromosome in a single gel with a highly reproducible restriction profile, providing clearly distinct and well-resolved DNA fragments.

Indexed as

Chromosomes, BacterialDNADNA, BacterialDNA Restriction EnzymesElectrophoresis, Gel, Pulsed-FieldGenotypeDNADNA, BacterialDNA Restriction EnzymesGenomic DNAGenotyping techniquePulse field gel electrophoresisRestriction enzyme

Identifiers

PMID25682374
PMCPMC4582012
OpenAlexW2475007798

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.