Evidence map›Paper›PMID 25682155›Full record

ArticleBiomaterials2015

Enhanced survival and engraftment of transplanted stem cells using growth factor sequestering hydrogels.

Amit K Jha, Kevin M Tharp, Jianqin Ye, Jorge L Santiago-Ortiz, Wesley M Jackson, Andreas Stahl, David V Schaffer, Yerem Yeghiazarians, Kevin E Healy

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomaterials, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.

0numbers the graph read from it
0cells of the map it votes in
52citing papers in PubMed
9.7field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

52 citing papers in PubMed, 119 citations in OpenAlex.

  1. Article
  2. Article
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  4. Angiogenesis: Biological Mechanisms and In Vitro Models.Annals of biomedical engineering · 2025
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  7. Cell reprogramming: methods, mechanisms and applications.Cell regeneration (London, England) · 2025
    Review
  8. Article
  9. Article
  10. Article
  11. Advancing Synthetic Hydrogels through Nature-Inspired Materials Chemistry.Advanced materials (Deerfield Beach, Fla.) · 2024
    Review
  12. Article
  13. Article
  14. Article
  15. Review
  16. Review
  17. Engineering approaches for cardiac organoid formation and their characterization.Translational research : the journal of laboratory and clinical medicine · 2022
    Review
  18. Review
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Amit K JhaDepartment of Bioengineering, University of California, Berkeley, CA 94720, USA; Department of Material Science and Engineering, University of California, Berkeley, CA 94720, USA.
Kevin M TharpDepartment of Nutritional Science and Toxicology, University of California, Berkeley, CA 94720, USA.
Jianqin YeDepartment of Medicine, University of California, San Francisco, CA 94143, USA.
Jorge L Santiago-OrtizDepartment of Chemical and Biomolecular Engineering, University of California, Berkeley, CA 94720, USA.
Wesley M JacksonDepartment of Bioengineering, University of California, Berkeley, CA 94720, USA.
Andreas StahlDepartment of Nutritional Science and Toxicology, University of California, Berkeley, CA 94720, USA.
David V SchafferDepartment of Bioengineering, University of California, Berkeley, CA 94720, USA; Department of Chemical and Biomolecular Engineering, University of California, Berkeley, CA 94720, USA.
Yerem YeghiazariansDepartment of Medicine, University of California, San Francisco, CA 94143, USA; Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California, San Francisco, CA 94143, USA; Cardiovascular Research Institute, University of California, San Francisco, CA 94143, USA.
Kevin E HealyDepartment of Bioengineering, University of California, Berkeley, CA 94720, USA; Department of Material Science and Engineering, University of California, Berkeley, CA 94720, USA. Electronic address: kehealy@berkeley.edu.
University of California, Berkeley · USUniversity of California, San Francisco · US

Funding

UCSF Liver Core CenterP30DK026743 · NIDDK · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI Holger Willenbring · 1986 to 2026
$30.7M
Pre-doctoral Training in Molecular and Biochemical NutritionT32DK061918 · NIDDK · UNIVERSITY OF CALIFORNIA BERKELEY · PI NAPOLI, JOSEPH L · 2002 to 2016
$2.6M
Enabling Technologies for hES Cell Self-RenewalR01HL096525 · NHLBI · UNIVERSITY OF CALIFORNIA BERKELEY · PI HEALY, KEVIN EDWARD · 2009 to 2013
$1.8M
Matrix assisted cell transplantation of brown fatR01DK101293 · NIDDK · UNIVERSITY OF CALIFORNIA BERKELEY · PI HEALY, KEVIN EDWARD, STAHL, ANDREAS · 2014 to 2017
$1.7M
NHLBI NIH HHS R01 HL096525NHLBI NIH HHS R01HL096525NIDDK NIH HHS P30 DK026743NIDDK NIH HHS R01 DK101293NIDDK NIH HHS T32 DK061918
6 · The paper itself

Abstract

We have generated a bioinspired tunable system of hyaluronic acid (HyA)-based hydrogels for Matrix-Assisted Cell Transplantation (MACT). With this material, we have independently evaluated matrix parameters such as adhesion peptide density, mechanical properties, and growth factor sequestering capacity, to engineer an environment that imbues donor cells with a milieu that promotes survival and engraftment with host tissues after transplantation. Using a versatile population of Sca-1(+)/CD45(-) cardiac progenitor cells (CPCs), we demonstrated that the addition of heparin in the HyA hydrogels was necessary to coordinate the presentation of TGFβ1 and to support the trophic functions of the CPCs via endothelial cell differentiation and vascular like tubular network formation. Presentation of exogenous TGFβ1 by binding with heparin improved differentiated CPC function by sequestering additional endogenously-produced angiogenic factors. Finally, we demonstrated that TGFβ1 and heparin-containing HyA hydrogels can promote CPC survival when implanted subcutaneously into murine hind-limbs and encouraged their participation in the ensuing neovascular response, which included blood vessels that had anastomosed with the host's blood vessels.

Indexed as

Stem Cell TransplantationAnimalsBinding SitesBiocompatible MaterialsCell AdhesionCell DifferentiationCell ProliferationCell SurvivalHeparinHyaluronic AcidHydrogelsIntercellular Signaling Peptides and ProteinsMiceNeovascularization, PathologicPeptidesStem CellsBiocompatible MaterialsHeparinHyaluronic AcidHydrogelsIntercellular Signaling Peptides and ProteinsPeptidesSulfhydryl CompoundsTransforming Growth Factor beta1Growth factor sequestrationHeparinHyaluronic acid hydrogelNeovascularizationStem cell transplantationTGFβ1

Identifiers

PMID25682155
PMCPMC4457539
OpenAlexW2037747858

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.