ArticleMolecular and cellular probes2015
Detection of enteroviruses and parechoviruses by a multiplex real-time RT-PCR assay.
Article in Molecular and cellular probes, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers, 1 of them a synthesis that pooled it.
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Who cites it
14 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Molecular and serological diagnosis of toxoplasmosis: a systematic review and meta-analysis.Revista do Instituto de Medicina Tropical de Sao Paulo · 2023Pooled it
- Enterovirus infections in children.Pediatric investigation · 2026Review
- Sentinel surveillance of acute febrile illness in a Brazilian Amazon region: laboratory findings and epidemiological implications.Revista do Instituto de Medicina Tropical de Sao Paulo · 2026Article
- Prevalence and epidemiology of enterovirus species in a pediatric oncology patient population.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2025Article
- Metagenomic detection of central nervous system infections missedby conventional testing.JCI insight · 2025Article
- Emergence of a New Pathogen: A Retrospective Study of HPeV-5 Central Nervous System Disease in Alberta, Canada.Viruses · 2024Article
- An Overview of the Characteristics, Pathogenesis, Epidemiology, and Detection of Human Enterovirus in the Arabian Gulf Region.Viruses · 2024Review
- Encephalopathy Caused by Human Parvovirus B19 Genotype 1 Associated withCurrent issues in molecular biology · 2023Article
- Metagenomics characterization of respiratory viral RNA pathogens in children under five years with severe acute respiratory infection in the Free State, South Africa.Journal of medical virology · 2023Article
- High rate of human enteroviruses among clinically suspected meningitis cases at selected Hospitals in Addis Ababa, Ethiopia.PloS one · 2021Article
- Viral Etiologies of Meningitis in Patients with Presumed Pyogenic Meningitis at University Hospitals in Ethiopia.Infection and drug resistance · 2021Article
- Primers and probe design and precision assessment of the real time RT-PCR assay in Coxsackievirus A10 and enterovirus detection.Data in brief · 2017Article
- Acute Flaccid Paralysis Associated with Novel Enterovirus C105.Emerging infectious diseases · 2015Article
- Detection of enterovirus D68 in Canadian laboratories.Journal of clinical microbiology · 2015Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Detection of all enteroviruses while excluding cross-detection of rhinoviruses is challenging because of sequence similarities in the commonly used conserved targets for molecular assays. In addition, simultaneous detection and differentiation of enteroviruses and parechoviruses would be beneficial because of a similar clinical picture presented by these viruses. A sensitive and specific real-time RT-PCR protocol that can address these clinical needs would be valuable to molecular diagnostic laboratories. Here we report a multiplex nucleic acid based assay using hydrolysis probes targeting the 5' non-translated region for the detection and differentiation of enteroviruses and parechoviruses without cross-detection of rhinoviruses. This assay has been shown to detect enteroviruses belonging to the different species in a variety of specimen types without detecting the different species of rhinoviruses. Laboratory validation shows the assay to be sensitive, specific, reproducible, easy to set up and uses generic cycling conditions. This assay can be implemented for diagnostic testing of patient samples in a high throughput fashion.
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