Evidence map›Paper›PMID 25681470›Full record

ArticleExperimental biology and medicine (Maywood, N.J.)2015

Hypoxia promotes HO-8910PM ovarian cancer cell invasion via Snail-mediated MT1-MMP upregulation.

Lijun Sun, Ping Lin, Zhuo Qin, Yusi Liu, Li-Li Deng, Changlian Lu

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PubMed Full text at PMC Publisher
In one paragraph

Article in Experimental biology and medicine (Maywood, N.J.), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed, 1 pooled it
0.2field-weighted citation impact, top 42% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 1 synthesis or guideline pooled it, 15 citations in OpenAlex.

  1. Pooled it
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Lijun SunDepartment of Biopharmaceutical Sciences, College of Pharmacy, Harbin Medical University,Harbin, Heilongjiang 150081, P. R. China.
Ping LinDepartment of Biochemistry and Molecular Biology, Harbin Medical University, Harbin, Heilongjiang 150081, P. R. China.
Zhuo QinDepartment of Biopharmaceutical Sciences, College of Pharmacy, Harbin Medical University,Harbin, Heilongjiang 150081, P. R. China.
Yusi LiuDepartment of Biopharmaceutical Sciences, College of Pharmacy, Harbin Medical University,Harbin, Heilongjiang 150081, P. R. China.
Li-Li DengDepartment of Oncology, the Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150081, P. R. China.
Changlian LuDepartment of Biopharmaceutical Sciences, College of Pharmacy, Harbin Medical University,Harbin, Heilongjiang 150081, P. R. China lclian@aliyun.com.
Harbin Medical University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The molecular mechanisms of ovarian cancer cell invasion under hypoxia remain unclear. Here we employed a 3D collagen model and chick chorioallantoic membrane (CAM) invasion assay to explore the influence of hypoxia on ovarian cancer cell invasion. Hypoxia (both 1% O2 and CoCl2 150 and 250 µM) induced HO-8910PM ovarian cancer cell invasion in 3D collagen and collagenolysis determined by hydroxyproline. Pretreatment with a hypoxia inducible factor-1α inhibitor, YC-1, or MMP inhibitor, GM6001, significantly inhibited 3D collagen invasion and degradation and cell proliferation. Hypoxia stimulated both mRNA and protein expressions of membrane-type 1 matrix metalloproteinase (MT1-MMP) and promoted MT1-MMP translocation to the cell surface in an YC-1 sensitive manner. MT1-siRNA transfection inhibited hypoxia-induced invasion, proliferation, and collagen degradation of cells in 3D collagen. Hypoxia stimulated Snail mRNA and protein expression as well as translocation to nucleus in an YC-1 sensitive manner. Overexpression of Snail with a recombinant plasmid in HO-8910PM cells resulted in an enhanced invasion in 3D collagen. Transfection with Snail-specific siRNA significantly decreased MT1-MMP expression and 3D collagen invasion. Hypoxia-treated cells significantly broke the upper CAM surface of 11-day-old chick embryos and infiltrated interstitial tissue, completely blocked in the presence of YC-1 or GM6001, or after MT1-MMP siRNA or Snail siRNA transfection. Together, these data suggest that hypoxia promotes HO-8910PM ovarian cancer cell traffic through 3D matrix via Snail-mediated MT1-MMP upregulation, a possible molecular mechanism of ovarian cancer cell invasion under hypoxia.

Indexed as

Gene Expression Regulation, NeoplasticAnimalsCell Culture TechniquesCell HypoxiaCell Line, TumorCell ProliferationChick EmbryoChorioallantoic MembraneCollagen Type IExtracellular MatrixFemaleHumansImmunohistochemistryIndazolesMatrix Metalloproteinase 14Neoplasm Invasiveness3-(5'-hydroxymethyl-2'-furyl)-1-benzylindazoleCollagen Type IIndazolesMatrix Metalloproteinase 14MMP14 protein, humanRNA, Small InterferingSnail Family Transcription FactorsTranscription FactorsHypoxiainvasionmembrane-type 1 matrix metalloproteinaseovarian cancerSnailthree-dimensional extracellular matrix

Identifiers

PMID25681470
PMCPMC4935300
OpenAlexW2010911851

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.