ArticleBiology open2015
Heterodimeric TALENs induce targeted heritable mutations in the crustacean Daphnia magna.
Article in Biology open, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 33 citations in OpenAlex.
- Optimization of mtDNA-targeted platinum TALENs for bi-directionally modifying heteroplasmy levels in patient-derived m.3243A>G-iPSCs.Molecular therapy. Nucleic acids · 2025Article
- Spatiotemporal control of transgene expression using an infrared laser in the crustacean Daphnia magna.Scientific reports · 2024Article
- Identification of gene isoforms and their switching events between male and female embryos of the parthenogenetic crustacean Daphnia magna.Scientific reports · 2024Article
- QTL study reveals candidate genes underlying host resistance in a Red Queen model system.PLoS genetics · 2023Article
- Review
- Production of genome-edited Daphnia for heavy metal detection by fluorescence.Scientific reports · 2020Article
- Roles of and cross-talk between ecdysteroid and sesquiterpenoid pathways in embryogenesis of branchiopod crustacean Daphnia magna.PloS one · 2020Article
- Generation of an oxoglutarate dehydrogenase knockout rat model and the effect of a high-fat diet.RSC advances · 2018Article
- Genomic integration and ligand-dependent activation of the human estrogen receptor α in the crustacean Daphnia magna.PloS one · 2018Article
- Sensory Ecology of Predator-Induced Phenotypic Plasticity.Frontiers in behavioral neuroscience · 2018Review
- Mapping the expression of the sex determining factor Doublesex1 in Daphnia magna using a knock-in reporter.Scientific reports · 2017Article
- Co-option of the bZIP transcription factor Vrille as the activator of Doublesex1 in environmental sex determination of the crustacean Daphnia magna.PLoS genetics · 2017Article
- Naming CRISPR alleles: endonuclease-mediated mutation nomenclature across species.Mammalian genome : official journal of the International Mammalian Genome Society · 2017Review
- Population Genomics ofGenetics · 2017Article
- CRISPR/Cas-mediated knock-in via non-homologous end-joining in the crustacean Daphnia magna.PloS one · 2017Article
- Editing the Neuronal Genome: a CRISPR View of Chromatin Regulation in Neuronal Development, Function, and Plasticity.The Yale journal of biology and medicine · 2016Review
- CRISPR/Cas9-Mediated Genome Editing and Mutagenesis ofG3 (Bethesda, Md.) · 2016Article
- TALEN-mediated knock-in via non-homologous end joining in the crustacean Daphnia magna.Scientific reports · 2016Article
- TALEN-mediated homologous recombination in Daphnia magna.Scientific reports · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transcription activator-like effector nucleases (TALENs) are artificial nucleases harboring a customizable DNA-binding domain and a FokI nuclease domain. The high specificity of the DNA-binding domain and the ease of design have enabled researchers to use TALENs for targeted mutagenesis in various organisms. Here, we report the development of TALEN-dependent targeted gene disruption in the crustacean Daphnia magna, the emerging model for ecological and toxicological genomics. First, a reporter transgene DsRed2 (EF1α-1::DsRed2) was targeted. Using the Golden Gate method with a GoldyTALEN scaffold, we constructed homodimeric and heterodimeric TALENs containing wild-type and ELD/KKR FokI domains. mRNAs that coded for either the customized homodimeric or heterodimeric TALENs were injected into one-cell-stage embryos. The high mortality of embryos injected with homodimeric TALEN mRNAs prevented us from detecting mutations. In contrast, embryos injected with heterodimeric TALEN mRNAs survived and 78%-87% of the adults lost DsRed2 fluorescence in a large portion of cells throughout the body. In addition, these adults produced non-fluorescent progenies, all of which carried mutations at the dsRed2 locus. We also tested heterodimeric TALENs targeted for the endogenous eyeless gene and found that biallelic mutations could be transmitted through germ line cells at a rate of up to 22%. Both somatic and heritable mutagenesis efficiencies of TALENs were higher than those of the CRISPR/Cas9 system that we recently developed. These results suggest that the TALEN system may efficiently induce heritable mutations into the target genes, which will further contribute to the progress of functional genomics in D. magna.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.