Evidence map›Paper›PMID 25681328›Full record

ArticlePlant physiology2015

Arabidopsis type I proton-pumping pyrophosphatase expresses strongly in phloem, where it is required for pyrophosphate metabolism and photosynthate partitioning.

Gaston A Pizzio, Julio Paez-Valencia, Aswad S Khadilkar, Kamesh Regmi, Araceli Patron-Soberano, Shangji Zhang, Jonathan Sanchez-Lares, Tara Furstenau, Jisheng Li, Concepcion Sanchez-Gomez and 4 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Plant physiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.

0numbers the graph read from it
0cells of the map it votes in
41citing papers in PubMed
17.1field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

41 citing papers in PubMed, 80 citations in OpenAlex.

  1. Article
  2. Overexpression ofGM crops & food · 2025
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  5. Review
  6. Overexpression of vacuolar HFrontiers in plant science · 2024
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  8. Review
  9. Article
  10. An Adapted Protocol for Quantitative Rhizosphere Acidification Assay.Methods in molecular biology (Clifton, N.J.) · 2023
    Article
  11. Article
  12. The HHorticulture research · 2021
    Article
  13. Review
  14. Plant Proton Pumps and Cytosolic pH-Homeostasis.Frontiers in plant science · 2021
    Review
  15. Review
  16. Article
  17. Lack of Vacuolar HFrontiers in plant science · 2020
    Article
  18. Review
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 5 institutions in 2 countries.

Gaston A PizzioSchool of Life Sciences, Arizona State University, Tempe, Arizona 852872 (G.A.P., J.P.-V., K.R., S.Z., J.S.-L., T.F., J.L., R.A.G.);Department of Botany, University of Wisconsin, Madison, Wisconsin 53706 (J.P.-V.);Department of Biological Sciences, University of North Texas, Denton, Texas 762031 (A.S.K., U.P.Y., B.G.A.);División de Biología Molecular, Laboratorio Nacional de Investigaciones en Nanociencias y Nanotecnología Instituto Potosino de Investigación Científica y Tecnológica, A.C. 78216 San Luis Potosí, Mexico (A.P.-S.); andDepartamento de Patología, Hospital Infantil de México, Federico Gómez, Mexico (C.S.-G., P.V.-M.).
Julio Paez-ValenciaSchool of Life Sciences, Arizona State University, Tempe, Arizona 852872 (G.A.P., J.P.-V., K.R., S.Z., J.S.-L., T.F., J.L., R.A.G.);Department of Botany, University of Wisconsin, Madison, Wisconsin 53706 (J.P.-V.);Department of Biological Sciences, University of North Texas, Denton, Texas 762031 (A.S.K., U.P.Y., B.G.A.);División de Biología Molecular, Laboratorio Nacional de Investigaciones en Nanociencias y Nanotecnología Instituto Potosino de Investigación Científica y Tecnológica, A.C. 78216 San Luis Potosí, Mexico (A.P.-S.); andDepartamento de Patología, Hospital Infantil de México, Federico Gómez, Mexico (C.S.-G., P.V.-M.).
Aswad S KhadilkarSchool of Life Sciences, Arizona State University, Tempe, Arizona 852872 (G.A.P., J.P.-V., K.R., S.Z., J.S.-L., T.F., J.L., R.A.G.);Department of Botany, University of Wisconsin, Madison, Wisconsin 53706 (J.P.-V.);Department of Biological Sciences, University of North Texas, Denton, Texas 762031 (A.S.K., U.P.Y., B.G.A.);División de Biología Molecular, Laboratorio Nacional de Investigaciones en Nanociencias y Nanotecnología Instituto Potosino de Investigación Científica y Tecnológica, A.C. 78216 San Luis Potosí, Mexico (A.P.-S.); andDepartamento de Patología, Hospital Infantil de México, Federico Gómez, Mexico (C.S.-G., P.V.-M.).
Kamesh RegmiSchool of Life Sciences, Arizona State University, Tempe, Arizona 852872 (G.A.P., J.P.-V., K.R., S.Z., J.S.-L., T.F., J.L., R.A.G.);Department of Botany, University of Wisconsin, Madison, Wisconsin 53706 (J.P.-V.);Department of Biological Sciences, University of North Texas, Denton, Texas 762031 (A.S.K., U.P.Y., B.G.A.);División de Biología Molecular, Laboratorio Nacional de Investigaciones en Nanociencias y Nanotecnología Instituto Potosino de Investigación Científica y Tecnológica, A.C. 78216 San Luis Potosí, Mexico (A.P.-S.); andDepartamento de Patología, Hospital Infantil de México, Federico Gómez, Mexico (C.S.-G., P.V.-M.).
Araceli Patron-SoberanoSchool of Life Sciences, Arizona State University, Tempe, Arizona 852872 (G.A.P., J.P.-V., K.R., S.Z., J.S.-L., T.F., J.L., R.A.G.);Department of Botany, University of Wisconsin, Madison, Wisconsin 53706 (J.P.-V.);Department of Biological Sciences, University of North Texas, Denton, Texas 762031 (A.S.K., U.P.Y., B.G.A.);División de Biología Molecular, Laboratorio Nacional de Investigaciones en Nanociencias y Nanotecnología Instituto Potosino de Investigación Científica y Tecnológica, A.C. 78216 San Luis Potosí, Mexico (A.P.-S.); andDepartamento de Patología, Hospital Infantil de México, Federico Gómez, Mexico (C.S.-G., P.V.-M.).
Shangji ZhangSchool of Life Sciences, Arizona State University, Tempe, Arizona 852872 (G.A.P., J.P.-V., K.R., S.Z., J.S.-L., T.F., J.L., R.A.G.);Department of Botany, University of Wisconsin, Madison, Wisconsin 53706 (J.P.-V.);Department of Biological Sciences, University of North Texas, Denton, Texas 762031 (A.S.K., U.P.Y., B.G.A.);División de Biología Molecular, Laboratorio Nacional de Investigaciones en Nanociencias y Nanotecnología Instituto Potosino de Investigación Científica y Tecnológica, A.C. 78216 San Luis Potosí, Mexico (A.P.-S.); andDepartamento de Patología, Hospital Infantil de México, Federico Gómez, Mexico (C.S.-G., P.V.-M.).
Jonathan Sanchez-LaresSchool of Life Sciences, Arizona State University, Tempe, Arizona 852872 (G.A.P., J.P.-V., K.R., S.Z., J.S.-L., T.F., J.L., R.A.G.);Department of Botany, University of Wisconsin, Madison, Wisconsin 53706 (J.P.-V.);Department of Biological Sciences, University of North Texas, Denton, Texas 762031 (A.S.K., U.P.Y., B.G.A.);División de Biología Molecular, Laboratorio Nacional de Investigaciones en Nanociencias y Nanotecnología Instituto Potosino de Investigación Científica y Tecnológica, A.C. 78216 San Luis Potosí, Mexico (A.P.-S.); andDepartamento de Patología, Hospital Infantil de México, Federico Gómez, Mexico (C.S.-G., P.V.-M.).
Tara FurstenauSchool of Life Sciences, Arizona State University, Tempe, Arizona 852872 (G.A.P., J.P.-V., K.R., S.Z., J.S.-L., T.F., J.L., R.A.G.);Department of Botany, University of Wisconsin, Madison, Wisconsin 53706 (J.P.-V.);Department of Biological Sciences, University of North Texas, Denton, Texas 762031 (A.S.K., U.P.Y., B.G.A.);División de Biología Molecular, Laboratorio Nacional de Investigaciones en Nanociencias y Nanotecnología Instituto Potosino de Investigación Científica y Tecnológica, A.C. 78216 San Luis Potosí, Mexico (A.P.-S.); andDepartamento de Patología, Hospital Infantil de México, Federico Gómez, Mexico (C.S.-G., P.V.-M.).
Jisheng LiSchool of Life Sciences, Arizona State University, Tempe, Arizona 852872 (G.A.P., J.P.-V., K.R., S.Z., J.S.-L., T.F., J.L., R.A.G.);Department of Botany, University of Wisconsin, Madison, Wisconsin 53706 (J.P.-V.);Department of Biological Sciences, University of North Texas, Denton, Texas 762031 (A.S.K., U.P.Y., B.G.A.);División de Biología Molecular, Laboratorio Nacional de Investigaciones en Nanociencias y Nanotecnología Instituto Potosino de Investigación Científica y Tecnológica, A.C. 78216 San Luis Potosí, Mexico (A.P.-S.); andDepartamento de Patología, Hospital Infantil de México, Federico Gómez, Mexico (C.S.-G., P.V.-M.).
Concepcion Sanchez-GomezSchool of Life Sciences, Arizona State University, Tempe, Arizona 852872 (G.A.P., J.P.-V., K.R., S.Z., J.S.-L., T.F., J.L., R.A.G.);Department of Botany, University of Wisconsin, Madison, Wisconsin 53706 (J.P.-V.);Department of Biological Sciences, University of North Texas, Denton, Texas 762031 (A.S.K., U.P.Y., B.G.A.);División de Biología Molecular, Laboratorio Nacional de Investigaciones en Nanociencias y Nanotecnología Instituto Potosino de Investigación Científica y Tecnológica, A.C. 78216 San Luis Potosí, Mexico (A.P.-S.); andDepartamento de Patología, Hospital Infantil de México, Federico Gómez, Mexico (C.S.-G., P.V.-M.).
Pedro Valencia-MayoralSchool of Life Sciences, Arizona State University, Tempe, Arizona 852872 (G.A.P., J.P.-V., K.R., S.Z., J.S.-L., T.F., J.L., R.A.G.);Department of Botany, University of Wisconsin, Madison, Wisconsin 53706 (J.P.-V.);Department of Biological Sciences, University of North Texas, Denton, Texas 762031 (A.S.K., U.P.Y., B.G.A.);División de Biología Molecular, Laboratorio Nacional de Investigaciones en Nanociencias y Nanotecnología Instituto Potosino de Investigación Científica y Tecnológica, A.C. 78216 San Luis Potosí, Mexico (A.P.-S.); andDepartamento de Patología, Hospital Infantil de México, Federico Gómez, Mexico (C.S.-G., P.V.-M.).
Umesh P YadavSchool of Life Sciences, Arizona State University, Tempe, Arizona 852872 (G.A.P., J.P.-V., K.R., S.Z., J.S.-L., T.F., J.L., R.A.G.);Department of Botany, University of Wisconsin, Madison, Wisconsin 53706 (J.P.-V.);Department of Biological Sciences, University of North Texas, Denton, Texas 762031 (A.S.K., U.P.Y., B.G.A.);División de Biología Molecular, Laboratorio Nacional de Investigaciones en Nanociencias y Nanotecnología Instituto Potosino de Investigación Científica y Tecnológica, A.C. 78216 San Luis Potosí, Mexico (A.P.-S.); andDepartamento de Patología, Hospital Infantil de México, Federico Gómez, Mexico (C.S.-G., P.V.-M.).
Brian G AyreSchool of Life Sciences, Arizona State University, Tempe, Arizona 852872 (G.A.P., J.P.-V., K.R., S.Z., J.S.-L., T.F., J.L., R.A.G.);Department of Botany, University of Wisconsin, Madison, Wisconsin 53706 (J.P.-V.);Department of Biological Sciences, University of North Texas, Denton, Texas 762031 (A.S.K., U.P.Y., B.G.A.);División de Biología Molecular, Laboratorio Nacional de Investigaciones en Nanociencias y Nanotecnología Instituto Potosino de Investigación Científica y Tecnológica, A.C. 78216 San Luis Potosí, Mexico (A.P.-S.); andDepartamento de Patología, Hospital Infantil de México, Federico Gómez, Mexico (C.S.-G., P.V.-M.).
Roberto A GaxiolaSchool of Life Sciences, Arizona State University, Tempe, Arizona 852872 (G.A.P., J.P.-V., K.R., S.Z., J.S.-L., T.F., J.L., R.A.G.);Department of Botany, University of Wisconsin, Madison, Wisconsin 53706 (J.P.-V.);Department of Biological Sciences, University of North Texas, Denton, Texas 762031 (A.S.K., U.P.Y., B.G.A.);División de Biología Molecular, Laboratorio Nacional de Investigaciones en Nanociencias y Nanotecnología Instituto Potosino de Investigación Científica y Tecnológica, A.C. 78216 San Luis Potosí, Mexico (A.P.-S.); andDepartamento de Patología, Hospital Infantil de México, Federico Gómez, Mexico (C.S.-G., P.V.-M.) roberto.gaxiola@asu.edu.
Arizona State University · USUniversity of North Texas · USHospital Infantil de México Federico Gómez · MXInstitute for Scientific and Technological Research · MXUniversity of Wisconsin–Madison · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Phloem loading is a critical process in plant physiology. The potential of regulating the translocation of photoassimilates from source to sink tissues represents an opportunity to increase crop yield. Pyrophosphate homeostasis is crucial for normal phloem function in apoplasmic loaders. The involvement of Arabidopsis (Arabidopsis thaliana) type I proton-pumping pyrophosphatase (AVP1) in phloem loading was analyzed at genetic, histochemical, and physiological levels. A transcriptional AVP1 promoter::GUS fusion revealed phloem activity in source leaves. Ubiquitous AVP1 overexpression (35S::AVP1 cassette) enhanced shoot biomass, photoassimilate production and transport, rhizosphere acidification, and expression of sugar-induced root ion transporter genes (POTASSIUM TRANSPORTER2 [KUP2], NITRATE TRANSPORTER2.1 [NRT2.1], NRT2.4, and PHOSPHATE TRANSPORTER1.4 [PHT1.4]). Phloem-specific AVP1 overexpression (Commelina Yellow Mottle Virus promoter [pCOYMV]::AVP1) elicited similar phenotypes. By contrast, phloem-specific AVP1 knockdown (pCoYMV::RNAiAVP1) resulted in stunted seedlings in sucrose-deprived medium. We also present a promoter mutant avp1-2 (SALK046492) with a 70% reduction of expression that did not show severe growth impairment. Interestingly, AVP1 protein in this mutant is prominent in the phloem. Moreover, expression of an Escherichia coli-soluble pyrophosphatase in the phloem (pCoYMV::pyrophosphatase) of avp1-2 plants resulted in severe dwarf phenotype and abnormal leaf morphology. We conclude that the Proton-Pumping Pyrophosphatase AVP1 localized at the plasma membrane of the sieve element-companion cell complexes functions as a synthase, and that this activity is critical for the maintenance of pyrophosphate homeostasis required for phloem function.

Indexed as

Gene Expression Regulation, PlantArabidopsisArabidopsis ProteinsDiphosphatesGene ExpressionGenes, ReporterHomeostasisInorganic PyrophosphataseMutationOrgan SpecificityPhenotypePhloemPlant LeavesPlant RootsPlants, Genetically ModifiedPlant ShootsArabidopsis ProteinsAVP1 protein, ArabidopsisDiphosphatesdiphosphoric acidInorganic PyrophosphataseSucrose

Identifiers

PMID25681328
PMCPMC4378163
OpenAlexW2073752784

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