ArticleApplied and environmental microbiology2015
Disparate independent genetic events disrupt the secondary metabolism gene perA in certain symbiotic Epichloë species.
Article in Applied and environmental microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 36 citations in OpenAlex.
- The Compatibility of theJournal of fungi (Basel, Switzerland) · 2026Article
- Morphological, Molecular, and Alkaloid Gene Profiling ofMicroorganisms · 2025Article
- Identification and Characterization of ThreeJournal of fungi (Basel, Switzerland) · 2025Article
- Discovery and Characterization ofMicroorganisms · 2024Article
- Identification of ThreeJournal of fungi (Basel, Switzerland) · 2022Article
- Cross-species transcriptomics identifies core regulatory changes differentiating the asymptomatic asexual and virulent sexual life cycles of grass-symbiotic Epichloë fungi.G3 (Bethesda, Md.) · 2022Article
- Dissection of the epoxyjanthitrem pathway inFrontiers in fungal biology · 2022Article
- Genetic Diversity of the Symbiotic FungusFrontiers in microbiology · 2021Article
- Lolium perenne apoplast metabolomics for identification of novel metabolites produced by the symbiotic fungus Epichloë festucae.The New phytologist · 2020Article
- Novel Antifungal Activity ofMicroorganisms · 2020Article
- Molecular mechanisms in grass-Epichloë interactions: towards endophyte driven farming to improve plant fitness and immunity.World journal of microbiology & biotechnology · 2020Review
- Efficient nonenzymatic cyclization and domain shuffling drive pyrrolopyrazine diversity from truncated variants of a fungal NRPS.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Transcriptome Analysis of Choke Stroma and Asymptomatic Inflorescence Tissues Reveals Changes in Gene Expression in BothMicroorganisms · 2019Article
- Infection Rates and Alkaloid Patterns of Different Grass Species with SystemicApplied and environmental microbiology · 2019Article
- Genetic modification of asexual Epichloë endophytes with the perA gene for peramine biosynthesis.Molecular genetics and genomics : MGG · 2019Article
- Chromosome rearrangements shape the diversification of secondary metabolism in the cyclosporin producing fungus Tolypocladium inflatum.BMC genomics · 2019Article
- Metabolism or behavior: explaining the performance of aphids on alkaloid-producing fungal endophytes in annual ryegrass (Lolium multiflorum).Oecologia · 2017Article
- Genomic Data Quality Impacts Automated Detection of Lateral Gene Transfer in Fungi.G3 (Bethesda, Md.) · 2017Article
- Comprehensive Analysis of Codon Usage Bias in SevenFrontiers in microbiology · 2017Article
- Endophytic Epichloë species and their grass hosts: from evolution to applications.Plant molecular biology · 2016Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
Peramine is an insect-feeding deterrent produced by Epichloë species in symbiotic association with C3 grasses. The perA gene responsible for peramine synthesis encodes a two-module nonribosomal peptide synthetase. Alleles of perA are found in most Epichloë species; however, peramine is not produced by many perA-containing Epichloë isolates. The genetic basis of these peramine-negative chemotypes is often unknown. Using PCR and DNA sequencing, we analyzed the perA genes from 72 Epichloë isolates and identified causative mutations of perA null alleles. We found nonfunctional perA-ΔR* alleles, which contain a transposon-associated deletion of the perA region encoding the C-terminal reductase domain, are widespread within the Epichloë genus and represent a prevalent mutation found in nonhybrid species. Disparate phylogenies of adjacent A2 and T2 domains indicated that the deletion of the reductase domain (R*) likely occurred once and early in the evolution of the genus, and subsequently there have been several recombinations between those domains. A number of novel point, deletion, and insertion mutations responsible for abolishing peramine production in full-length perA alleles were also identified. The regions encoding the first and second adenylation domains (A1 and A2, respectively) were common sites for such mutations. Using this information, a method was developed to predict peramine chemotypes by combining PCR product size polymorphism analysis with sequencing of the perA adenylation domains.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.