ArticleApplied and environmental microbiology2015
High-throughput microfluidic method to study biofilm formation and host-pathogen interactions in pathogenic Escherichia coli.
Article in Applied and environmental microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed, 41 citations in OpenAlex.
- A Predictive Computational Framework forPathogens (Basel, Switzerland) · 2026Article
- New Methodologies as Opportunities in the Study of Bacterial Biofilms, Including Food-Related Applications.Microorganisms · 2025Review
- Veillonella dispar and V. atypica increased the growth of Listeria monocytogenes in liquid culture and biofilm conditions.PloS one · 2025Article
- Diverse polysaccharide production and biofilm formation abilities of clinical Klebsiella pneumoniae.NPJ biofilms and microbiomes · 2024Article
- Diverse polysaccharide production and biofilm formation abilities of clinical Klebsiella pneumoniae.Research square · 2024Article
- Biofilm Formation byMicroorganisms · 2023Article
- Effectiveness of Bacteriophages against Biofilm-Forming Shiga-ToxigenicFoods (Basel, Switzerland) · 2023Article
- How to study biofilms: technological advancements in clinical biofilm research.Frontiers in cellular and infection microbiology · 2023Review
- Antibiofilm Properties of Antiseptic Agents Used onInternational journal of molecular sciences · 2022Article
- Real-time monitoring of mono- and dual-species biofilm formation and eradication using microfluidic platform.Scientific reports · 2022Article
- New Adapted In Vitro Technology to Evaluate Biofilm Formation and Antibiotic Activity Using Live Imaging under Flow Conditions.Diagnostics (Basel, Switzerland) · 2021Article
- A Selection of Platforms to Evaluate Surface Adhesion and Biofilm Formation in Controlled Hydrodynamic Conditions.Microorganisms · 2021Review
- Microtiter plate assays to assess antibiofilm activity against bacteria.Nature protocols · 2021Article
- Antibacterial and Antibiofilm Activities of Novel Antimicrobial Peptides against Multidrug-Resistant EnterotoxigenicInternational journal of molecular sciences · 2021Article
- Article
- Role of CpxR in Biofilm Development: Expression of Key Fimbrial, O-Antigen and Virulence Operons ofInternational journal of molecular sciences · 2019Article
- Testing Anti-Biofilm Polymeric Surfaces: Where to Start?International journal of molecular sciences · 2019Review
- Machine-assisted cultivation and analysis of biofilms.Scientific reports · 2019Article
- Live Cell Analysis of Shear Stress on Pseudomonas aeruginosa Using an Automated Higher-Throughput Microfluidic System.Journal of visualized experiments : JoVE · 2019Article
- Options and Limitations in Clinical Investigation of Bacterial Biofilms.Clinical microbiology reviews · 2018Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Biofilm formation and host-pathogen interactions are frequently studied using multiwell plates; however, these closed systems lack shear force, which is present at several sites in the host, such as the intestinal and urinary tracts. Recently, microfluidic systems that incorporate shear force and very small volumes have been developed to provide cell biology models that resemble in vivo conditions. Therefore, the objective of this study was to determine if the BioFlux 200 microfluidic system could be used to study host-pathogen interactions and biofilm formation by pathogenic Escherichia coli. Strains of various pathotypes were selected to establish the growth conditions for the formation of biofilms in the BioFlux 200 system on abiotic (glass) or biotic (eukaryotic-cell) surfaces. Biofilm formation on glass was observed for the majority of strains when they were grown in M9 medium at 30 °C but not in RPMI medium at 37 °C. In contrast, HRT-18 cell monolayers enhanced binding and, in most cases, biofilm formation by pathogenic E. coli in RPMI medium at 37 °C. As a proof of principle, the biofilm-forming ability of a diffusely adherent E. coli mutant strain lacking AIDA-I, a known mediator of attachment, was assessed in our models. In contrast to the parental strain, which formed a strong biofilm, the mutant formed a thin biofilm on glass or isolated clusters on HRT-18 monolayers. In conclusion, we describe a microfluidic method for high-throughput screening that could be used to identify novel factors involved in E. coli biofilm formation and host-pathogen interactions under shear force.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.