ArticleInternational urology and nephrology2015
Laser-capture microdissection for analysis of cell type-specific gene expression of muscarinic receptor subtypes in the rat bladder with cyclophosphamide-induced cystitis.
Article in International urology and nephrology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 11 citations in OpenAlex.
- Animal models of interstitial cystitis/bladder pain syndrome.Frontiers in physiology · 2023Review
- The Effect of Acetylcysteine on Renal Function in Experimental Models of Cyclophosphamide-and Ifosfamide-Induced Cystitis.Current urology · 2020Article
- Uroprotective effect of pantoprazole against cyclophosphamide-induced cystitis in mice.Supportive care in cancer : official journal of the Multinational Association of Supportive Care in Cancer · 2019Article
- Unveiling of miRNA Expression Patterns in Purkinje Cells During Development.Cerebellum (London, England) · 2017Article
- Morphological Plasticity of Emerging Purkinje Cells in Response to Exogenous VEGF.Frontiers in molecular neuroscience · 2017Article
- Blocking PAR2 Alleviates Bladder Pain and Hyperactivity via TRPA1 Signal.Translational neuroscience · 2016Article
- Blocking mammalian target of rapamycin alleviates bladder hyperactivity and pain in rats with cystitis.Molecular pain · 2016Article
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Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
Abstract
purposeThis study examined whether the laser-capture microdissection (LCM) method can achieve separation of urothelial cells from detrusor cells or superficial urothelial cells from intermediate/basal urothelial cells, using α-smooth muscle actin (SMA) and cytokeratin 20 (CK20). In addition, we investigated the changes in expression of muscarinic receptors in laser-captured urothelial and detrusor cells in rats with chronic cystitis.
methodsFemale SD rats were injected with cyclophosphamide (75 mg/kg) intraperitoneally at day 1, 4, 7 and 10 to induce chronic cystitis. Saline was injected in the same protocol for controls. Bladder specimens were cut at 8 μm thickness, fixed in 70% ethanol and lightly stained by hematoxylin and eosin, and then superficial urothelium, intermediate/basal urothelium and detrusor muscles were laser-captured separately. Real-time PCR was performed to examine expressions of α-SMA, CK20, muscarinic 2 receptors (M2R) and muscarinic 3 receptors (M3R).
resultsThe expression of α-SMA mRNA in detrusor muscle cells was 200 times higher than that in urothelial cells in controls. CK20 mRNA expression in apical urothelial cells was 55 times more than that in detrusor muscle and four times more than that in intermediate/basal urothelial cells. Expressions of M2R and M3R mRNA were increased in urothelial cells and decreased in detrusor muscles following chronic cystitis.
conclusionsThe LCM could be useful for tissue collection of detrusor muscle and different layers of urothelial cells with minimal contamination of other cell types, and cell type-specific changes in molecular expression could accurately be analyzed. Increased expression of urothelial MR might enhance urothelial-afferent interactions to induce bladder overactivity/pain conditions associated with bladder inflammation.
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