ArticleMolecular & cellular proteomics : MCP2015
Acetylproteomic analysis reveals functional implications of lysine acetylation in human spermatozoa (sperm).
Article in Molecular & cellular proteomics : MCP, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 68 citations in OpenAlex.
- Effects of Sodium Butyrate on Sperm Function and Protein Acetylation in Fresh and Frozen-Thawed Boar Spermatozoa.Animals : an open access journal from MDPI · 2026Article
- Lysine malonylation regulates human sperm motility.Communications biology · 2026Article
- Spermatozoa Proteins Involved in ROS Generation and Antioxidant Defense Are Differentially Acetylated in Idiopathic Infertility.Antioxidants (Basel, Switzerland) · 2025Article
- Analysis of Semen Proteomic Differences Among Three Genotypes of FecB Rams in Duolang Sheep.Genes · 2025Article
- Scriptaid is a prospective agent for improving human asthenozoospermic sample quality and fertilization rate in vitro.Asian journal of andrology · 2024Article
- Citrate Promotes Nitric Oxide Production during Human Sperm Capacitation.Antioxidants (Basel, Switzerland) · 2024Article
- The compound YK 3-237 promotes pig sperm capacitation-related events.Veterinary research communications · 2024Article
- Mechanisms That Protect Mammalian Sperm from the Spontaneous Acrosome Reaction.International journal of molecular sciences · 2023Review
- Cryopreservation Induces Acetylation of Metabolism-Related Proteins in Boar Sperm.International journal of molecular sciences · 2023Article
- Functional prediction and profiling of exosomal circRNAs derived from seminal plasma for the diagnosis and treatment of oligoasthenospermia.Experimental and therapeutic medicine · 2022Article
- Effects of Heat Stress on Motion Characteristics and Metabolomic Profiles of Boar Spermatozoa.Genes · 2022Article
- ZDHHC19 Is Dispensable for Spermatogenesis, but Is Essential for Sperm Functions in Mice.International journal of molecular sciences · 2021Article
- Functions and Mechanisms of Lysine Glutarylation in Eukaryotes.Frontiers in cell and developmental biology · 2021Review
- Post-Translational Modification Analysis of VDAC1 in ALS-SOD1 Model Cells Reveals Specific Asparagine and Glutamine Deamidation.Antioxidants (Basel, Switzerland) · 2020Article
- Functional Analysis of Differentially Expressed Acetylated Spermatozoal Proteins in Infertile Men with Unilateral and Bilateral Varicocele.International journal of molecular sciences · 2020Article
- Review
- Redox Signaling from Mitochondria: Signal Propagation and Its Targets.Biomolecules · 2020Review
- Metabolite Support of Long-Term Storage of Sperm in the Spermatheca of Honeybee (Frontiers in physiology · 2020Article
- Protective Effects of Oridonin on Acute Liver Injury via Impeding Posttranslational Modifications of Interleukin-1 Receptor-Associated Kinase 4 (IRAK4) in the Toll-Like Receptor 4 (TLR4) Signaling Pathway.Mediators of inflammation · 2019Article
- Proteomic Changes in Human Sperm During SequentialFrontiers in cell and developmental biology · 2019Article
Corrections and comments
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Authors and funding
12 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Male infertility is a medical condition that has been on the rise globally. Lysine acetylation of human sperm, an essential posttranslational modification involved in the etiology of sperm abnormality, is not fully understood. Therefore, we first generated a qualified pan-anti-acetyllysine monoclonal antibody to characterize the global lysine acetylation of uncapacitated normal human sperm with a proteomics approach. With high enrichment ratios that were up to 31%, 973 lysine-acetylated sites that matched to 456 human sperm proteins, including 671 novel lysine acetylation sites and 205 novel lysine-acetylated proteins, were identified. These proteins exhibited conserved motifs XXXKYXXX, XXXKFXXX, and XXXKHXXX, were annotated to function in multiple metabolic processes, and were localized predominantly in the mitochondrion and cytoplasmic fractions. Between the uncapacitated and capacitated sperm, different acetylation profiles in regard to functional proteins involved in sperm capacitation, sperm-egg recognition, sperm-egg plasma fusion, and fertilization were observed, indicating that acetylation of functional proteins may be required during sperm capacitation. Bioinformatics analysis revealed association of acetylated proteins with diseases and drugs. Novel acetylation of voltage-dependent anion channel proteins was also found. With clinical sperm samples, we observed differed lysine acetyltransferases and lysine deacetylases expression between normal sperm and abnormal sperm of asthenospermia or necrospermia. Furthermore, with sperm samples impaired by epigallocatechin gallate to mimic asthenospermia, we observed that inhibition of sperm motility was partly through the blockade of voltage-dependent anion channel 2 Lys-74 acetylation combined with reduced ATP levels and mitochondrial membrane potential. Taken together, we obtained a qualified pan-anti-acetyllysine monoclonal antibody, analyzed the acetylproteome of uncapacitated human sperm, and revealed associations between functional protein acetylation and sperm functions.
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