ArticleOrganic & biomolecular chemistry2015
Bichromophoric dyes for wavelength shifting of dye-protein fluoromodules.
Article in Organic & biomolecular chemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
4 citing papers in PubMed, 10 citations in OpenAlex.
- Design, synthesis and application in biological imaging of a novel red fluorescent dye based on a rhodanine derivative.RSC advances · 2020Article
- Antibody-Linked Fluorogen-Activating Proteins for Antigen Detection and Cell Ablation.Bioconjugate chemistry · 2019Article
- Breaking the color barrier - a multi-selective antibody reporter offers innovative strategies of fluorescence detection.Journal of cell science · 2017Article
- Dark dyes-bright complexes: fluorogenic protein labeling.Current opinion in chemical biology · 2015Review
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
Dye-protein fluoromodules consist of fluorogenic dyes and single chain antibody fragments that form brightly fluorescent noncovalent complexes. This report describes two new bichromophoric dyes that extend the range of wavelengths of excitation or emission of existing fluoromodules. In one case, a fluorogenic thiazole orange (TO) was attached to an energy acceptor dye, Cy5. Upon binding to a protein that recognizes TO, red emission due to efficient energy transfer from TO to Cy5 replaces the green emission observed for monochromophoric TO bound to the same protein. Separately, TO was attached to a coumarin that serves as an energy donor. The same green emission is observed for coumarin-TO and TO bound to a protein, but efficient energy transfer allows violet excitation of coumarin-TO, versus longer wavelength, blue excitation of monochromophoric TO. Both bichromophores exhibit low nanomolar KD values for their respective proteins, >95% energy transfer efficiency and high fluorescence quantum yields.
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Registered trials
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