ArticlePloS one2015
Sufficient numbers of early germ cells are essential for female sex development in zebrafish.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 48 citations in OpenAlex.
- Functional Sex Reversal in Adult Gonochoristic Teleosts is Mediated by Ovarian Germline Stem Cell Plasticity and Somatic Cell Transdifferentiation.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Efficient recovery of zebrafish maternal-zygotic mutants from in vivo expanded and cryopreserved germline stem cells.Communications biology · 2026Article
- Zglp-1 is a novel essential transcriptional regulator for sex reversal in zebrafish.Marine life science & technology · 2025Article
- Migration of primordial germ cells and their relationship of PGCs with sex development in transgenic germline-specific fluorescent freshwater angelfish (Pterophyllum scalare).Scientific reports · 2025Article
- Adamts9 is required for the development of primary ovarian follicles and maintenance of female sex in zebrafish†.Biology of reproduction · 2024Article
- New insights into the all-testis differentiation in zebrafish with compromised endogenous androgen and estrogen synthesis.PLoS genetics · 2024Article
- Estrogen Signaling Inhibits the Expression ofInternational journal of molecular sciences · 2024Article
- Production of all-male non-transgenic zebrafish by conditional primordial germ cell ablation.Fish physiology and biochemistry · 2023Article
- Zebrafish gonad mutant models reveal neuroendocrine mechanisms of brain sexual dimorphism and male mating behaviors of different brain regions.Biology of sex differences · 2023Article
- The use ofSystems biology in reproductive medicine · 2023Article
- brca2-mutant zebrafish exhibit context- and tissue-dependent alterations in cell phenotypes and response to injury.Scientific reports · 2022Article
- Becoming female: Ovarian differentiation from an evolutionary perspective.Frontiers in cell and developmental biology · 2022Review
- Metalloproteases in gonad formation and ovulation.General and comparative endocrinology · 2021Review
- Delay in primordial germ cell migration in adamts9 knockout zebrafish.Scientific reports · 2021Article
- Zebrafish dazl regulates cystogenesis and germline stem cell specification during the primordial germ cell to germline stem cell transition.Development (Cambridge, England) · 2021Article
- Characterization of the Zebrafish Cell Landscape at Single-Cell Resolution.Frontiers in cell and developmental biology · 2021Article
- A Transient Hermaphroditic Stage in Early Male Gonadal Development in Little Yellow Croaker,Frontiers in endocrinology · 2020Article
- Article
- Noncanonical translation via deadenylated 3' UTRs maintains primordial germ cells.Nature chemical biology · 2018Article
- Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The sex determination for zebrafish is controlled by a combination of genetic and environmental factors. The determination of sex in zebrafish has been suggested to rely on a mechanism that is affected by germ cell-derived signals. To begin our current study, a simplified and efficient germ cell-specific promoter of the dead end (dnd) gene was identified. Utilizing the metrodinazole (MTZ)/ bacterial nitroreductase (NTR) system for inducible germ cell ablation, several stable Tg (dnd:NTR-EGFP(-3'UTR)) and Tg (dnd:NTR-EGFP(+3'UTR)) zebrafish lines were then generated with the identified promoter. A thorough comparison of the expression patterns and tissue distributions of endogenous dnd and ntr-egfp transcripts in vivo revealed that the identified 2032-bp zebrafish dnd promoter can recapitulate dnd expression faithfully in stable transgenic zebrafish. The correlation between the levels of the germ cell-derived signals and requirement for maintaining the female fate has been also explored with different durations of the MTZ treatments. Our results revealed the decreasing ratios of female presented in the treated transgenic group are fairly associated with the reducing levels of the early germ cell-derived signals. After the juvenile transgenic fish treated with 5 mM MTZ for 20 days, all MTZ-treated transgenic fish exclusively developed into males with subfertilities. Taken together, our results identified here a simplified and efficient dnd promoter, and provide clear evidence indicating that it was not the presence but the sufficiency of signals derived from germ cells that is essential for female sex development in zebrafish. Our model also provides a unique system for sex control in zebrafish studies.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.