ArticleJournal of clinical biochemistry and nutrition2015
Hydrogen peroxide generated by xanthine/xanthine oxidase system represses the proliferation of colorectal cancer cell line Caco-2.
Article in Journal of clinical biochemistry and nutrition, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 33 citations in OpenAlex.
- Protective effects of Tinospora crispa and Tinospora cordifolia extracts against methylglyoxal-induced oxidative stress and apoptosis in hFOB 1.19 cells: ELISA-based evaluation and molecular docking insights with antioxidant enzymes.BMC complementary medicine and therapies · 2026Article
- XOR-mediated tumor-selective nanomaterial therapy: achieving tumor and immune cell dual regulation through intracellular ROS generation.Journal of nanobiotechnology · 2026Article
- Arginase 2 attenuates ulcerative colitis by antioxidant effects of spermidine.Journal of gastroenterology · 2024Article
- Liquid-Phase Adsorption Behavior of β-D-Glucooligosaccharides When Using Activated Carbon for Separation, and the Antioxidant Stress Activity of Purified Fractions.Foods (Basel, Switzerland) · 2024Article
- Study of the effect of keap1 on oxidative stress in human umbilical cord mesenchymal stem cells.Molecular biology reports · 2024Article
- Fusobacterium nucleatum-triggered purine metabolic reprogramming drives tumorigenesis in head and neck carcinoma.Discover oncology · 2023Article
- Thiol-disulphide independent in-cell trapping for the identification of peroxiredoxin 2 interactors.Redox biology · 2021Article
- A coupled enzyme assay for detection of selenium-binding protein 1 (SELENBP1) methanethiol oxidase (MTO) activity in mature enterocytes.Redox biology · 2021Article
- ROS systems are a new integrated network for sensing homeostasis and alarming stresses in organelle metabolic processes.Redox biology · 2020Review
- Effect of α2‑macroglobulin in the early stage of jaw osteoradionecrosis.International journal of oncology · 2020Article
- Article
- Effect of Manitoba-Grown Red-Osier Dogwood Extracts on Recovering Caco-2 Cells from HAntioxidants (Basel, Switzerland) · 2019Article
- Nitric oxide represses the proliferation of Caco-2 cells by inducing S-GInternational journal of physiology, pathophysiology and pharmacology · 2019Article
- Heterogeneous Fenton Reaction Enabled Selective Colon Cancerous Cell Treatment.Scientific reports · 2018Article
- Comparison of the Functional Activities of Xanthine Oxidases Isolated from Microorganisms and from Cow's Milk.Doklady. Biochemistry and biophysics · 2018Article
- Protective effects of α‑2‑macroglobulin on human bone marrow mesenchymal stem cells in radiation injury.Molecular medicine reports · 2018Article
- Potential Mechanisms Connecting Purine Metabolism and Cancer Therapy.Frontiers in immunology · 2018Review
- A marine bio-functional lipid, fucoxanthinol, attenuates human colorectal cancer stem-like cell tumorigenicity and sphere formation.Journal of clinical biochemistry and nutrition · 2017Article
- Neuroprotective effects of Liriope platyphylla extract against hydrogen peroxide-induced cytotoxicity in human neuroblastoma SH-SY5Y cells.BMC complementary and alternative medicine · 2015Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The twin character of reactive oxygen species is substantiated by a growing body of evidence that reactive oxygen species within cells act as inducers and accelerators of the oncogenic phenotype of cancer cells, while reactive oxygen species can also induce cancer cell death and can therefore function as anti-tumorigenic species. The aim of this study was to assess a possible influence of xanthine/xanthine oxidase on the proliferation of colorectal cancer cell line Caco-2. xanthine/xanthine oxidase (2.5 µM/0.25 mU/ml-25 µM/2.5 mU/ml) dose-dependently inhibited the proliferation of Caco-2 cells. Experiments utilizing reactive oxygen species scavengers (superoxide dismutase, catalase and mannitol) and exogenous hydrogen peroxide revealed a major role of hydrogen peroxide in the xanthine/xanthine oxidase effect. Investigations utilizing annexin V-fluorescein/PI assay using flow cytometry, and the lactate dehydrogenase extracellular release assay indicated that hydrogen peroxide induced necrosis, but not apoptosis, in Caco-2 cells. These results suggest that hydrogen peroxide generated by xanthine/xanthine oxidase has the potential to suppress colorectal cancer cell proliferation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.