ArticleBiotechnology and bioengineering2015
Automated detection of whole-cell mitochondrial motility and its dependence on cytoarchitectural integrity.
Article in Biotechnology and bioengineering, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 38 citations in OpenAlex.
- RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells.FEBS open bio · 2026Article
- Nellie: automated organelle segmentation, tracking and hierarchical feature extraction in 2D/3D live-cell microscopy.Nature methods · 2025Article
- Mitochondrial Functioning: Front and Center in Defining Psychosomatic Mechanisms of Allostasis in Health and Disease.Methods in molecular biology (Clifton, N.J.) · 2025Review
- Caffeine, MitoQ, and GABA Prophylaxis of Mitochondrial Dysfunction Induced in Human Pulmonary Cells by Normobaric-Hyperoxia and Hyperbaric-Hyperoxia.Oxidative medicine and cellular longevity · 2025Article
- Artificial Intelligence for Organelle Segmentation in Live-Cell Imaging.Research (Washington, D.C.) · 2025Review
- Three-dimensional object geometry of mitochondria-associated signal: 3-D analysis pipeline for two-photon image stacks of cerebrovascular endothelial mitochondria.American journal of physiology. Heart and circulatory physiology · 2024Article
- Mitochondrial networks through the lens of mathematics.Physical biology · 2023Review
- MitoTNT: Mitochondrial Temporal Network Tracking for 4D live-cell fluorescence microscopy data.PLoS computational biology · 2023Article
- How cytoskeletal proteins regulate mitochondrial energetics in cell physiology and diseases.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2022Review
- Mitochondrial dynamics involves molecular and mechanical events in motility, fusion and fission.Frontiers in cell and developmental biology · 2022Review
- Automated segmentation and tracking of mitochondria in live-cell time-lapse images.Nature methods · 2021Article
- Crosstalk between mechanotransduction and metabolism.Nature reviews. Molecular cell biology · 2021Review
- Ex vivo use of cell-permeable succinate prodrug attenuates mitochondrial dysfunction in blood cells obtained from carbon monoxide-poisoned individuals.American journal of physiology. Cell physiology · 2020Article
- Prophylaxis of mitochondrial dysfunction caused by cellular decompression from hyperbaric exposure.Mitochondrion · 2020Article
- Mitochondrial networking in human blood cells with application in acute care illnesses.Mitochondrion · 2019Article
- Acute decompression following simulated dive conditions alters mitochondrial respiration and motility.American journal of physiology. Cell physiology · 2018Article
- Force Spectrum Microscopy Using Mitochondrial Fluctuations of Control and ATP-Depleted Cells.Biophysical journal · 2018Article
- Translational Application of Measuring Mitochondrial Functions in Blood Cells Obtained from Patients with Acute Poisoning.Journal of medical toxicology : official journal of the American College of Medical Toxicology · 2018Article
- Mitochondrial dynamics and respiration within cells with increased open pore cytoskeletal meshes.Biology open · 2017Article
- Connecting mitochondrial dynamics and life-or-death events via Bcl-2 family proteins.Neurochemistry international · 2017Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Current methodologies used for mitochondrial motility analysis tend to either overlook individual mitochondrial tracks or analyze only peripheral mitochondria instead of mitochondria in all regions of the cell. Furthermore, motility analysis of an individual mitochondrion is usually quantified by establishing an arbitrary threshold for "directed" motion. In this work, we created a custom, publicly available computational algorithm based on a previously published approach (Giedt et al., 2012. Ann Biomed Eng 40:1903-1916) in order to characterize the distribution of mitochondrial movements at the whole-cell level, while still preserving information about single mitochondria. Our technique is easy to use, robust, and computationally inexpensive. Images are first pre-processed for increased resolution, and then individual mitochondria are tracked based on object connectivity in space and time. When our method is applied to microscopy fields encompassing entire cells, we reveal that the mitochondrial net distances in fibroblasts follow a lognormal distribution within a given cell or group of cells. The ability to model whole-cell mitochondrial motility as a lognormal distribution provides a new quantitative paradigm for comparing mitochondrial motility in naïve and treated cells. We further demonstrate that microtubule and microfilament depolymerization shift the lognormal distribution in directions which indicate decreased and increased mitochondrial movement, respectively. These findings advance earlier work on neuronal axons (Morris and Hollenbeck, 1993. J Cell Sci 104:917-927) by relating them to a different cell type, applying them on a global scale, and automating measurement of mitochondrial motility in general.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.