ArticleInternational urogynecology journal2015
Changes in hyperpolarization-activated cyclic nucleotide-gated channel expression and activity in bladder interstitial cells of Cajal from rats with detrusor overactivity.
Article in International urogynecology journal, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 22 citations in OpenAlex.
- Ion Channels as Therapeutic Targets in Overactive Bladder: A Narrative Review of Mechanisms, Translational Evidence, and Current Gaps.Lower urinary tract symptoms · 2026Review
- Pharmacological Targeting of Hyperpolarization-Activated Cyclic Nucleotide-Gated Cation Channels on Bladder Afferent Sensory Transmission.Lower urinary tract symptoms · 2025Article
- Acupuncture for Women with Overactive Bladder: Perspective of Traditional Chinese Medicine and Related Mechanism.International journal of general medicine · 2023Review
- The hyperpolarization-activated, cyclic nucleotide-gated channel resides on myocytes in mouse bladders and contributes to adrenergic-induced detrusor relaxation.American journal of physiology. Regulatory, integrative and comparative physiology · 2022Article
- Imatinib Mesylate Reduces Voiding Frequency in Female Mice With Acute Cyclophosphamide-Induced Cystitis.Frontiers in systems neuroscience · 2022Article
- Imatinib Mesylate Reduces Neurotrophic Factors and pERK and pAKT Expression in Urinary Bladder of Female Mice With Cyclophosphamide-Induced Cystitis.Frontiers in systems neuroscience · 2022Article
- Aging Changes in Bladder Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels Are Associated With Increasing Heterogeneity of Adrenergic/Mucosal Influence on Detrusor Control in the Mouse.The journals of gerontology. Series A, Biological sciences and medical sciences · 2021Article
- HCN as a Mediator of Urinary Homeostasis: Age-Associated Changes in Expression and Function in Adrenergic Detrusor Relaxation.The journals of gerontology. Series A, Biological sciences and medical sciences · 2019Article
- Decreased hyperpolarization-activated cyclic nucleotide-gated channels are involved in bladder dysfunction associated with spinal cord injury.International journal of molecular medicine · 2018Article
- The role of HCN channels in peristaltic dysfunction in human ureteral tuberculosis.International urology and nephrology · 2018Article
- Hyperpolarization-Activated Cyclic Nucleotide-Gated Non-selective (HCN) Ion Channels Regulate Human and Murine Urinary Bladder Contractility.Frontiers in physiology · 2018Article
- Minimal Invasive Cystometry and Intra-Abdominal Pressure Assessments in Rodents: A Novel Animal Study.Medical science monitor : international medical journal of experimental and clinical research · 2017Article
- Cyclophosphamide-induced HCN1 channel upregulation in interstitial Cajal-like cells leads to bladder hyperactivity in mice.Experimental & molecular medicine · 2017Article
- Distribution of interstitial cells of Cajal in the bladders of fetal rats with retinoic acid induced myelomeningocele.Turkish journal of urology · 2016Article
- Characterization of the role of HCN channels in β3-adrenoceptor mediated rat bladder relaxation.Bladder (San Francisco, Calif.)Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
introduction and hypothesisTo investigate changes in the expression and function of the hyperpolarization-activated cyclic nucleotide-gated (HCN) channels, interstitial cells of Cajal (ICCs) were isolated from detrusor overactivity (DO) rats induced with partial bladder outlet obstruction (PBOO). We hypothesized that increased expression of HCN channels in ICCs would enhance the excitability of ICCs and bladder in DO rats.
methodsForty adult female Sprague-Dawley rats were randomly assigned to control and DO groups. The expression of HCN isoforms in the rat bladders was detected using reverse transcription-polymerase chain reaction (RT-PCR). Whole-cell patch-clamp techniques and laser confocal microscopy were used to explore the effects of the HCN channel blocker ZD7288 on Ih current and intracellular calcium levels ([Ca(2+)]i) in freshly isolated ICCs. The effect of ZD7288 on bladder contraction was evaluated using a bladder smooth muscle strip test.
resultsThe results of RT-PCR showed that HCN1-4 isoforms increased expression in the DO group compared with the control group. The current density of Ih in the bladder ICCs was increased. A moderate concentration of ZD7288 (50 μmol/L) significantly decreased the [Ca(2+)]i levels in freshly isolated ICCs from sham and DO bladders. The smooth strip tests indicated that ZD7288 (50 μmol/L) suppressed the amplitude of smooth muscle strip contractions in the sham and DO bladders.
conclusionsAll HCN channel isoforms were highly expressed in bladders from the DO group, which was correlated with increased Ih currents in DO ICCs, suggesting that the HCN channels might play an important role in the pathological processes of DO.
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