ArticleMolecular plant pathology2015
A PCR assay for the quantification of growth of the oomycete pathogen Hyaloperonospora arabidopsidis in Arabidopsis thaliana.
Article in Molecular plant pathology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 32 citations in OpenAlex.
- RT-qPCR-Based Estimation ofJournal of fungi (Basel, Switzerland) · 2026Article
- Downy mildew disease-suppressive soils transmit a protective core microbiome to the phyllosphere.The ISME journal · 2025Article
- Quantification of the fungal pathogen Didymella segeticola in Camellia sinensis using a DNA-based qRT-PCR assay.Plant methods · 2024Article
- Revealing real-time 3D in vivo pathogen dynamics in plants by label-free optical coherence tomography.Nature communications · 2024Article
- Obligate biotroph downy mildew consistently induces near-identical protective microbiomes in Arabidopsis thaliana.Nature microbiology · 2023Article
- Rapid molecular assay for the evaluation of clove essential oil antifungal activity against wheat common bunt.Frontiers in plant science · 2023Article
- Infection timing affects Fusarium poae colonization of bread wheat spikes and mycotoxin accumulation in the grain.Journal of the science of food and agriculture · 2022Article
- A split green fluorescent protein system to enhance spatial and temporal sensitivity of translating ribosome affinity purification.The Plant journal : for cell and molecular biology · 2022Article
- Article
- The Histological, Effectoromic, and Transcriptomic Analyses ofInternational journal of molecular sciences · 2020Article
- Promising Perspectives for Detection, Identification, and Quantification of Plant Pathogenic Fungi and Oomycetes through Targeting Mitochondrial DNA.International journal of molecular sciences · 2020Review
- Development of a DNA-based real-time PCR assay for the quantification ofPlant methods · 2020Article
- Mechanisms of glacial-to-future atmospheric COThe New phytologist · 2018Article
- Identification of Lipid Markers ofFrontiers in plant science · 2018Article
- A DNA-based real-time PCR assay for robust growth quantification of the bacterial pathogenPlant methods · 2016Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The accurate quantification of disease severity is important for the assessment of host-pathogen interactions in laboratory or field settings. The interaction between Arabidopsis thaliana and its naturally occurring downy mildew pathogen, Hyaloperonospora arabidopsidis (Hpa), is a widely used reference pathosystem for plant-oomycete interactions. Current methods for the assessment of disease severity in the Arabidopsis-Hpa interaction rely on measurements at the terminal stage of pathogen development; namely, visual counts of spore-producing structures or the quantification of spore production with a haemocytometer. These assays are useful, but do not offer sensitivity for the robust quantification of small changes in virulence or the accurate quantification of pathogen growth prior to the reproductive stage. Here, we describe a quantitative real-time polymerase chain reaction (qPCR) assay for the monitoring of Hpa growth in planta. The protocol is rapid, inexpensive and can robustly distinguish small changes in virulence. We used this assay to investigate the dynamics of early Hpa mycelial growth and to demonstrate the proof of concept that this assay could be used in screens for novel oomycete growth inhibitors.
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