ArticleProceedings of the National Academy of Sciences of the United States of America2015
Visualizing monolayers with a water-soluble fluorophore to quantify adsorption, desorption, and the double layer.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed, 33 citations in OpenAlex.
- From relaxation to buckling: A continuum elastic framework connecting surface instabilities of highly compressed lipid thin films.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Boron Cluster Anions Dissolve En Masse in Lipids Causing Membrane Expansion and Thinning.Angewandte Chemie (International ed. in English) · 2024Article
- Article
- Extracting the Heterogeneous 3D Structure of Molecular Films Using Higher Dimensional SFG Microscopy.The journal of physical chemistry letters · 2024Article
- Spiral packing and chiral selectivity in model membranes probed by phase-resolved sum-frequency generation microscopy.Nature communications · 2024Article
- Evolution of interfacial mechanics of lung surfactant mimics progression of acute respiratory distress syndrome.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Annexin A5 stabilizes matrix vesicle-biomimetic lipid membranes: unravelling a new role of annexins in calcification.European biophysics journal : EBJ · 2023Article
- Dilatational and shear rheology of soluble and insoluble monolayers with a Langmuir trough.Journal of colloid and interface science · 2023Article
- Microtensiometer for Confocal Microscopy Visualization of Dynamic Interfaces.Journal of visualized experiments : JoVE · 2022Article
- Spontaneous evolution of equilibrium morphology in phospholipid-cholesterol monolayers.Science advances · 2022Article
- Inflammation product effects on dilatational mechanics can trigger the Laplace instability and acute respiratory distress syndrome.Soft matter · 2020Article
- Interfacial rheology and direct imaging reveal domain-templated network formation in phospholipid monolayers penetrated by fibrinogen.Soft matter · 2019Article
- Interfacial Stress in the Development of Biologics: Fundamental Understanding, Current Practice, and Future Perspective.The AAPS journal · 2019Article
- Evolution and mechanics of mixed phospholipid fibrinogen monolayers.Journal of the Royal Society, Interface · 2018Article
- Protons at the speed of sound: Predicting specific biological signaling from physics.Scientific reports · 2016Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
Contrast in confocal microscopy of phase-separated monolayers at the air-water interface can be generated by the selective adsorption of water-soluble fluorescent dyes to disordered monolayer phases. Optical sectioning minimizes the fluorescence signal from the subphase, whereas convolution of the measured point spread function with a simple box model of the interface provides quantitative assessment of the excess dye concentration associated with the monolayer. Coexisting liquid-expanded, liquid-condensed, and gas phases could be visualized due to differential dye adsorption in the liquid-expanded and gas phases. Dye preferentially adsorbed to the liquid-disordered phase during immiscible liquid-liquid phase coexistence, and the contrast persisted through the critical point as shown by characteristic circle-to-stripe shape transitions. The measured dye concentration in the disordered phase depended on the phase composition and surface pressure, and the dye was expelled from the film at the end of coexistence. The excess concentration of a cationic dye within the double layer adjacent to an anionic phospholipid monolayer was quantified as a function of subphase ionic strength, and the changes in measured excess agreed with those predicted by the mean-field Gouy-Chapman equations. This provided a rapid and noninvasive optical method of measuring the fractional dissociation of lipid headgroups and the monolayer surface potential.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.