ArticlePloS one2015
A reverse transcription loop-mediated isothermal amplification assay optimized to detect multiple HIV subtypes.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed.
- Rapid, low-cost, and portable molecular detection of HIV-1 subtype B and HCV 1a directly from blood.Microbiology spectrum · 2026Article
- Development of a Highly Multiplexed RT-LAMP Assay for Coverage of Genetic Sequence Diversity.Analytical chemistry · 2025Article
- Compact highly sensitive photothermal RT-LAMP chip for simultaneous multidisease detection.Science advances · 2024Article
- Field-applicable simultaneous multiplex LAMP assay for screening HBV and HCV co-infection in a single tube.BMC infectious diseases · 2024Article
- Development of a LAMP-Based Diagnostic for the Detection of Multiple HIV-1 Strains.Biosensors · 2024Article
- Vivid COVID-19 LAMP is an ultrasensitive, quadruplexed test using LNA-modified primers and a zinc ion and 5-Br-PAPS colorimetric detection system.Communications biology · 2023Article
- Rapid and visual identification of HIV-1 using reverse transcription loop-mediated isothermal amplification integrated with a gold nanoparticle-based lateral flow assay platform.Frontiers in microbiology · 2023Article
- Microfluidic Devices for HIV Diagnosis and Monitoring at Point-of-Care (POC) Settings.Biosensors · 2022Review
- Fingerpick Blood-Based Nucleic Acid Testing on A USB Interfaced Device towards HIV self-testing.Biosensors & bioelectronics · 2022Article
- Review
- A Mismatch-tolerant RT-LAMP Method for Molecular Diagnosis of Highly Variable Viruses.Bio-protocol · 2019Article
- Point-of-Care HIV Viral Load Testing: an Essential Tool for a Sustainable Global HIV/AIDS Response.Clinical microbiology reviews · 2019Review
- Classification of Multiple DNA Dyes Based on Inhibition Effects on Real-Time Loop-Mediated Isothermal Amplification (LAMP): Prospect for Point of Care Setting.Frontiers in microbiology · 2019Article
- A Mismatch-Tolerant Reverse Transcription Loop-Mediated Isothermal Amplification Method and Its Application on Simultaneous Detection of All Four Serotype of Dengue Viruses.Frontiers in microbiology · 2019Article
- Loop-mediated isothermal amplification (LAMP): a versatile technique for detection of micro-organisms.Journal of applied microbiology · 2018Review
- A Multifunctional Reactor with Dry-Stored Reagents for Enzymatic Amplification of Nucleic Acids.Analytical chemistry · 2018Article
- Miniaturized devices for point of care molecular detection of HIV.Lab on a chip · 2017Review
- Instrument-Free Point-of-Care Molecular Detection of Zika Virus.Analytical chemistry · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
Abstract
Diagnostic methods for detecting and quantifying HIV RNA have been improving, but efficient methods for point-of-care analysis are still needed, particularly for applications in resource-limited settings. Detection based on reverse-transcription loop-mediated isothermal amplification (RT-LAMP) is particularly useful for this, because when combined with fluorescence-based DNA detection, RT-LAMP can be implemented with minimal equipment and expense. Assays have been developed to detect HIV RNA with RT-LAMP, but existing methods detect only a limited subset of HIV subtypes. Here we report a bioinformatic study to develop optimized primers, followed by empirical testing of 44 new primer designs. One primer set (ACeIN-26), targeting the HIV integrase coding region, consistently detected subtypes A, B, C, D, and G. The assay was sensitive to at least 5000 copies per reaction for subtypes A, B, C, D, and G, with Z-factors of above 0.69 (detection of the minor subtype F was found to be unreliable). There are already rapid and efficient assays available for detecting HIV infection in a binary yes/no format, but the rapid RT-LAMP assay described here has additional uses, including 1) tracking response to medication by comparing longitudinal values for a subject, 2) detecting of infection in neonates unimpeded by the presence of maternal antibody, and 3) detecting infection prior to seroconversion.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.