Interaction between fatty acid synthase and human epidermal growth receptor 2 (HER2) in osteosarcoma cells.

Xin Hua Long, Kai Zhao, Guo Mei Zhang, Yang Zhou, Rong Ping Zhou, Zhi Li Liu, Zhi Hong Zhang

Open access · greenAbstract read

In one paragraph

Article in International journal of clinical and experimental pathology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact, top 100% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 4 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Xin Hua LongDepartment of Orthopedics, The First Affiliated Hospital of Nanchang University Jiangxi, P.R. China.

Kai ZhaoDepartment of Orthopedics, The First Affiliated Hospital of Nanchang University Jiangxi, P.R. China.

Guo Mei ZhangDepartment of Orthopedics, The Second Affiliated Hospital of Nanchang University Jiangxi, P. R. China.

Yang ZhouDepartment of Orthopedics, The First Affiliated Hospital of Nanchang University Jiangxi, P.R. China.

Rong Ping ZhouDepartment of Orthopedics, The Second Affiliated Hospital of Nanchang University Jiangxi, P. R. China.

Zhi Li LiuDepartment of Orthopedics, The First Affiliated Hospital of Nanchang University Jiangxi, P.R. China.

Zhi Hong ZhangDepartment of Orthopedics, The First Affiliated Hospital of Nanchang University Jiangxi, P.R. China.

Nanchang University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Elevated expression of fatty acid synthase (FASN) and human epidermal growth factor receptor-2 (HER2) are observed in human osteosarcoma (OS). The aim in this study is to investigate a possible connection between FASN expression and the activity of HER2. The immunohistochemistry staining was conducted on 24 OS specimens from patients, which revealed a significant positive correlation between FASN and HER2 as well as p-HER2 protein expression. Furthermore, the human OS cell lines MG-63 and U2-OS were treated with FASN-specific RNAi Plasmid or Lapatinib (an inhibitor of HER2). The mRNA of HER2 and FASN was measured using RT-PCR. Western blot was performed to detect the protein expression of HER2, p-HER2 and FASN. The results demonstrated that HER2 modulates FASN expression, inhibition of FASN resulted in down-regulation of HER2 and p-HER2 protein in OS cells. Our findings suggested that there was positive feedback regulation between FASN and HER2 expression and phosphorylation in OS cells.

Indexed as

Blotting, WesternErb-b2 Receptor Tyrosine KinasesFatty Acid Synthase, Type IGene Expression Regulation, NeoplasticHumansImmunohistochemistryOsteosarcomaReal-Time Polymerase Chain ReactionERBB2 protein, humanErb-b2 Receptor Tyrosine KinasesFASN protein, humanFatty Acid Synthase, Type IFASNHER2Osteosarcomapositive feedback regulation

Identifiers

PMID25674245

PMCPMC4313995

OpenAlexW15815970

What OpenQuestion holds

Textmetadata

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.