ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2015
Speed and sensitivity of phototransduction in Drosophila depend on degree of saturation of membrane phospholipids.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 63 citations in OpenAlex.
- Insect Lipidomics: Advances, Applications, and Physiological Insights.Advances in experimental medicine and biology · 2026Review
- Review of sensory systems deployed by epidermal keratinocytes.Frontiers in cell and developmental biology · 2025Review
- Piezo1 and tissue fibrosis: insights into its role and potential for modulation.Burns & trauma · 2025Review
- Omega-3 fatty acids protect cartilage from acute injurie by reducing the mechanical sensitivity of chondrocytes.Journal of orthopaedic surgery and research · 2024Article
- Eye proteome of Drosophila melanogaster.Proteomics · 2024Article
- Lipidome Unsaturation Affects the Morphology and Proteome of theJournal of proteome research · 2024Article
- Unlocking cellular traffic jams: olive oil-mediated rescue of CNG mutant channels.Frontiers in pharmacology · 2024Article
- Beyond Fluidity: The Role of Lipid Unsaturation in Membrane Function.Cold Spring Harbor perspectives in biology · 2023Review
- Review
- Linoleic acid improves PIEZO2 dysfunction in a mouse model of Angelman Syndrome.Nature communications · 2023Article
- Retinal TRP channels: Cell-type-specific regulators of retinal homeostasis and multimodal integration.Progress in retinal and eye research · 2023Review
- Genetic- and diet-induced ω-3 fatty acid enrichment enhances TRPV4-mediated vasodilation in mice.Cell reports · 2022Article
- Vitamin A Deficiency Alters the Phototransduction Machinery and Distinct Non-Vision-Specific Pathways in theBiomolecules · 2022Article
- Biophysical physiology of phosphoinositide rapid dynamics and regulation in living cells.The Journal of general physiology · 2022Article
- The Role of Membrane Lipids in Light-Activation ofBiomolecules · 2022Review
- Vitamin A deficiency affects gene expression in the Drosophila melanogaster head.G3 (Bethesda, Md.) · 2021Article
- Control of Neuronal Excitability by Cell Surface Receptor Density and Phosphoinositide Metabolism.Frontiers in pharmacology · 2021Article
- Hydroxylated sphingolipid biosynthesis regulates photoreceptor apical domain morphogenesis.The Journal of cell biology · 2020Article
- PE homeostasis rebalanced through mitochondria-ER lipid exchange prevents retinal degeneration in Drosophila.PLoS genetics · 2020Article
- Intracellular acidification facilitates receptor-operated TRPC4 activation through PLCδ1 in a CaThe Journal of physiology · 2020Article
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Authors and funding
9 authors at 4 institutions in 2 countries.
Funding
Abstract
Drosophila phototransduction is mediated via a G-protein-coupled PLC cascade. Recent evidence, including the demonstration that light evokes rapid contractions of the photoreceptors, suggested that the light-sensitive channels (TRP and TRPL) may be mechanically gated, together with protons released by PLC-mediated PIP2 hydrolysis. If mechanical gating is involved we predicted that the response to light should be influenced by altering the physical properties of the membrane. To achieve this, we used diet to manipulate the degree of saturation of membrane phospholipids. In flies reared on a yeast diet, lacking polyunsaturated fatty acids (PUFAs), mass spectrometry showed that the proportion of polyunsaturated phospholipids was sevenfold reduced (from 38 to ∼5%) but rescued by adding a single species of PUFA (linolenic or linoleic acid) to the diet. Photoreceptors from yeast-reared flies showed a 2- to 3-fold increase in latency and time to peak of the light response, without affecting quantum bump waveform. In the absence of Ca(2+) influx or in trp mutants expressing only TRPL channels, sensitivity to light was reduced up to ∼10-fold by the yeast diet, and essentially abolished in hypomorphic G-protein mutants (Gαq). PLC activity appeared little affected by the yeast diet; however, light-induced contractions measured by atomic force microscopy or the activation of ectopic mechanosensitive gramicidin channels were also slowed ∼2-fold. The results are consistent with mechanosensitive gating and provide a striking example of how dietary fatty acids can profoundly influence sensory performance in a classical G-protein-coupled signaling cascade.
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Registered trials
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